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The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds
Agriculture, 14 (2024), 4; 556. https://doi.org/10.3390/agriculture14040556
Bošnjak Mihovilović, Anita; Kereša, Snježana; Lazarević, Boris; Topolovec Pintarić, Snježana; Martinko, Katarina; Marković, Zvjezdana; Turkalj, Katarina; Habuš Jerčić, Ivanka

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Bošnjak Mihovilović, A., Kereša, S., Lazarević, B., Topolovec Pintarić, S., Martinko, K., Marković, Z. ... Habuš Jerčić, I. (2024). The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds. Agriculture, 14. (4). doi: 10.3390/agriculture14040556

Bošnjak Mihovilović, Anita, et al. "The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds." Agriculture, vol. 14, br. 4, 2024. https://doi.org/10.3390/agriculture14040556

Bošnjak Mihovilović, Anita, Snježana Kereša, Boris Lazarević, Snježana Topolovec Pintarić, Katarina Martinko, Zvjezdana Marković, Katarina Turkalj i Ivanka Habuš Jerčić. "The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds." Agriculture 14, br. 4 (2024). https://doi.org/10.3390/agriculture14040556

Bošnjak Mihovilović, A., et al. (2024) 'The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds', Agriculture, 14(4). doi: 10.3390/agriculture14040556

Bošnjak Mihovilović A, Kereša S, Lazarević B, Topolovec Pintarić S, Martinko K, Marković Z, i sur.. The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds. Agriculture [Internet]. 31.03.2024. [pristupljeno 06.01.2025.];14(4). doi: 10.3390/agriculture14040556

A. Bošnjak Mihovilović, et al., "The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds", Agriculture, vol. 14, br. 4, Ožujak 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:204:875201. [Citirano: 06.01.2025.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:204:875201
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Naslov (engleski)The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds
AutorAnita Bošnjak Mihovilović
AutorSnježana Kereša
AutorBoris Lazarević
AutorSnježana Topolovec Pintarić
AutorKatarina Martinko
AutorZvjezdana Marković
AutorKatarina Turkalj
AutorIvanka Habuš Jerčić
Autorova ustanovaSveučilište u Zagrebu
Agronomski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOTEHNIČKE ZNANOSTI
Poljoprivreda (agronomija)
Sažetak (engleski)
In vitro plants that are free of pathogens are crucial for biotechnological breeding methods. The present study investigates the effects of sterilization with sodium hypochlorite (NaClO) and the addition of Plant Preservative Mixture (PPMTM) to the growth medium on pathogen elimination, germination, and seedling development of the winter wheat (Triticum aestivum L.) variety Mara. The sterilization treatments differed in the duration of seed sterilization in 4% NaClO and the PPM concentration added to the growth medium. Pathogenic fungi of the genera Aspergillus, Cladosporium, Penicillium, and Bipolaris were completely eliminated when the seeds were sterilized in NaClO and placed on growth media with the addition of PPM. Extending the duration of the sterilization treatment with NaClO to 50 min reduced Fusarium contamination, while the interaction between the 50 min sterilization treatment with NaClO and the addition of PPM to the growth medium reduced Alternaria contamination. Our results suggest that PPM could complement sterilization procedures with NaClO in the introduction of highly infected wheat seeds in vitro. Seed germination was not affected by sterilization with NaClO or by the addition of PPM. However, PPM at a concentration of 4 mL L−1 had a negative effect on seedling development.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaAgriculture
Brojčani podacivol. 14, br. 4, 556
e-ISSN2077-0472
DOIhttps://doi.org/10.3390/agriculture14040556
URN:NBNurn:nbn:hr:204:875201
Datum objave publikacije2024-03-31
URL dokumentahttps://www.mdpi.com/2077-0472/14/4/556
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-12-04 08:42:12