prikaz prve stranice dokumenta The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds
  Download
PDF 1.29 MB
Scientific paper - Original scientific paper
The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds
Agriculture, 14 (2024), 4; 556. https://doi.org/10.3390/agriculture14040556
Bošnjak Mihovilović, Anita; Kereša, Snježana; Lazarević, Boris; Topolovec Pintarić, Snježana; Martinko, Katarina; Marković, Zvjezdana; Turkalj, Katarina; Habuš Jerčić, Ivanka

Cite this document

Bošnjak Mihovilović, A., Kereša, S., Lazarević, B., Topolovec Pintarić, S., Martinko, K., Marković, Z. ... Habuš Jerčić, I. (2024). The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds. Agriculture, 14. (4). doi: 10.3390/agriculture14040556

Bošnjak Mihovilović, Anita, et al. "The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds." Agriculture, vol. 14, no. 4, 2024. https://doi.org/10.3390/agriculture14040556

Bošnjak Mihovilović, Anita, Snježana Kereša, Boris Lazarević, Snježana Topolovec Pintarić, Katarina Martinko, Zvjezdana Marković, Katarina Turkalj and Ivanka Habuš Jerčić. "The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds." Agriculture 14, no. 4 (2024). https://doi.org/10.3390/agriculture14040556

Bošnjak Mihovilović, A., et al. (2024) 'The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds', Agriculture, 14(4). doi: 10.3390/agriculture14040556

Bošnjak Mihovilović A, Kereša S, Lazarević B, Topolovec Pintarić S, Martinko K, Marković Z, and sur.. The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds. Agriculture [Internet]. 2024 March 31 [cited 2025 January 10];14(4). doi: 10.3390/agriculture14040556

A. Bošnjak Mihovilović, et al., "The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds", Agriculture, vol. 14, no. 4, March 2024. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:204:875201. [Accessed: 10 January 2025]

Metadata
Title (english)The Use of Sodium Hypochlorite and Plant Preservative Mixture Significantly Reduces Seed-Borne Pathogen Contamination When Establishing In Vitro Cultures of Wheat (Triticum aestivum L.) Seeds
AuthorAnita Bošnjak Mihovilović
AuthorSnježana Kereša
AuthorBoris Lazarević
AuthorSnježana Topolovec Pintarić
AuthorKatarina Martinko
AuthorZvjezdana Marković
AuthorKatarina Turkalj
AuthorIvanka Habuš Jerčić
Author's institutionUniversity of Zagreb
Faculty of Agriculture
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Abstract (english)
In vitro plants that are free of pathogens are crucial for biotechnological breeding methods. The present study investigates the effects of sterilization with sodium hypochlorite (NaClO) and the addition of Plant Preservative Mixture (PPMTM) to the growth medium on pathogen elimination, germination, and seedling development of the winter wheat (Triticum aestivum L.) variety Mara. The sterilization treatments differed in the duration of seed sterilization in 4% NaClO and the PPM concentration added to the growth medium. Pathogenic fungi of the genera Aspergillus, Cladosporium, Penicillium, and Bipolaris were completely eliminated when the seeds were sterilized in NaClO and placed on growth media with the addition of PPM. Extending the duration of the sterilization treatment with NaClO to 50 min reduced Fusarium contamination, while the interaction between the 50 min sterilization treatment with NaClO and the addition of PPM to the growth medium reduced Alternaria contamination. Our results suggest that PPM could complement sterilization procedures with NaClO in the introduction of highly infected wheat seeds in vitro. Seed germination was not affected by sterilization with NaClO or by the addition of PPM. However, PPM at a concentration of 4 mL L−1 had a negative effect on seedling development.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleAgriculture
Numberingvol. 14, no. 4, 556
e-ISSN2077-0472
DOIhttps://doi.org/10.3390/agriculture14040556
URN:NBNurn:nbn:hr:204:875201
Publication2024-03-31
Document URLhttps://www.mdpi.com/2077-0472/14/4/556
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-12-04 08:42:12