Primjena molekularnih analiza u determinaciji mikrobiote fecesa divljih vrsta pčela

master's thesis

• Title (english): Applied molecular analysis regarding microbiotic fecal residue of wild bee species
• Author: Marija Andrijana Galešić
• Mentor: Darija Lemić (mentor)
• Committee member: Darija Lemić (predsjednik povjerenstva)
• Committee member: Lidija Svečnjak (član povjerenstva)
• Committee member: Mirna Mrkonjić Fuka (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Agricultural Zoology); Zagreb
• Defense date and country: 2023-09-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Pčele kao glavni biljni oprašivači imaju ključnu ulogu u održavanju ravnoteže cjelokupnog
ekosustava. Solitarne pčele su nerijetko s u kontaktu sa medonosnim pčelama kao zadružnim
kukcima te mogu međusobno utjecati na sastav crijevne mikrobiote koja utječe na zdravlje
jedinki, ali i cijele zajednice. Provedbom DNA metabarkodiranja uzoraka fecesa triju vrsta
solitarnih pčela (Hoplitis adunca, Osmia bicornis, Megachile sculpturalis) utvrđene su
prehrambene preferencije i prisutnost mikroorganizama u crijevnoj mikrobioti jedinki. Biljne
preferencije prilikom ishrane ukazuju na biljke roda Echium sp., Prunus sp. i Ranunculus sp.
Analizom bakteriološkog sastava crijevne mikrobiote utvrđena je prisutnost bakterije
Melissococcus plutonius te prisutnost bakterija Bradyrhizobium sp. i Clostridium sp. Potrebna
su daljnja istraživanja solitarnih pčela i njihovih prehrambenih i ekoloških navika kako bi se
osigurao opstanak i poboljšanje broja njihovih populacija kao jednih od najvažnijih oprašivača
u prirodi.

Abstract (english)

Bees, as primary plant pollinators, play a crucial role in maintaining the balance of the entire
ecosystem. Solitary bees often come into contact with honeybees as social insects, and they
can mutually influence the composition of the gut microbiota, which impacts the health of
individuals as well as the entire community. By conducting DNA metabarcoding of fecal
samples from three species of solitary bees (Hoplitis adunca, Osmia bicornis, Megachile
sculpturalis), dietary preferences and the presence of microorganisms in the gut microbiota
of individuals were determined. Plant preferences during feeding point to plants of the genus
Echium sp., Prunus sp., and Ranunculus sp. Through analysis of the bacterial composition of
the gut microbiota, the presence of the bacterium Melissococcus plutonius was established,
along with the presence of bacteria Bradyrhizobium sp. and Clostridium sp. Further research
into the dietary and ecological habits of solitary bees is necessary to ensure the survival and
enhancement of their populations as one of the most important pollinators in nature.

• Keywords: solitarne pčele; feces; DNA metabarkodiranje
• Keywords (english): solitary bees; faeces; DNA metabarcoding
• Language: croatian
• URN:NBN: urn:nbn:hr:204:234675
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-21 10:18:40