Zastupljenost i raširenost gospodarski važnih virusa tikvica (Cucurbita pepo L.) i tikvi (Cucurbita sp.) u Hrvatskoj

Stručni rad - Stručni rad

• Naslov (engleski): Occurrence and distribution of economically important viruses of zucchini (Cucurbita pepo L.) and pumpkin (Cucurbita sp.) in Croatia
• Autor: Dario Ivić
• Autor: Jasna Milanović
• Autor: Lea Mihaljevski Boltek
• Autor: Adrijana Novak
• Autor: Darko Vončina
• Autorova ustanova: Sveučilište u Zagrebu; Agronomski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOTEHNIČKE ZNANOSTI; Poljoprivreda (agronomija)

Sažetak (hrvatski)

Posljednjih nekoliko godina, virusne bolesti tikvica i tikvi u Hrvatskoj javljaju se u sve jačem intenzitetu i uzrokuju gubitke u proizvodnji. Cilj rada bio je utvrditi prisutnost virusa mozaika krastavca (CMV), virusa žutog mozaika tikvice (ZYMV) i virusa mozaika lubenice (WMV) na tim kulturama. Tijekom 2019. godine, na 18 lokacija (polja na otvorenom) u šest županija provedeni su vizualni pregledi te je ukupno sakupljeno 43 simptomatičnih uzorka tikvice i 39 uzoraka tikve u svrhu laboratorijske analize na prisutnost CMV, ZYMV i WMV. Analiza je provedena imunoenzimskim testom DAS-ELISA korištenjem komercijalnih seroloških kompleta. Od 82 uzorka, CMV je potvrđen u njih 55 (67%), ZYMV u 50 (61%), a WMV u 10 uzoraka (12%). Pojedinačna zaraza s jednim od tri istraživana virusa utvrđena je u ukupno 38 uzoraka (46%). Mješovite zaraze s dva virusa zabilježene su u 36 uzoraka (44%): 32 uzorka (39%) s kombinacijom CMV + ZYMV te četiri uzorka (5%) s kombinacijom CMV + WMV. Mješovita zaraza sa sva tri virusa (CMV + ZYMV + WMV) utvrđena je u dva uzorka. U šest uzoraka (7%) nije zabilježen niti jedan od tri istraživana virusa. U osam nasada utvrđena je prisutnost jednog virusa, u devet nasada prisutnost dva virusa, a u jednom od 18 pregledanih nasada utvrđena je prisutnost sva tri istraživana virusa.

Sažetak (engleski)

Viral diseases of zucchini and pumpkin have become more severe in recent years, causing significant yield losses. The objective of this study was to determine the presence of cucumber mosaic virus (CMV), zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus (WMV) in these crops. In 2019, a survey was conducted at 18 sites (open fields) in six counties. Fortythree symptomatic samples of zucchini and 39 symptomatic samples of pumpkin were collected and tested for the presence of CMV, ZYMV and WMV. Samples were tested with commercial serological assays, using doubleantibody sandwich ELISA kits. Out of 82 samples analysed, CMV was detected in 55 (67%), ZYMV in 50 (61%), and WMV in 10 samples (12%). Single viral infection was detected in 38 samples (46%). Mixed infections with two viruses were recorded in 36 samples (44%), CMV + ZYMV combination in 32 samples (39%), and CMV + WMV combination in four samples (5%). Triple viral infection was detected in two samples, while six samples (7%) were determined to be free of all three viruses in the testes. One virus species was recorded at eight locations, two viruses at nine locations, while all three viruses were determined in one out of 18 sampled fields.

• Ključne riječi (hrvatski): Cucurbita pepo L.; virus mozaika krastavca (CMV); virus žutog mozaika tikvice (ZYMV); virus mozaika lubenice (WMV); ELISA
• Ključne riječi (engleski): Cucurbita pepo L..; cucumber mosaic virus (CMV); zucchini yellow mosaic virus (ZYMV); watermelon mosaic virus (WMV); ELISA
• Jezik: hrvatski
• Vrsta publikacije: Stručni rad - Stručni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Agronomski glasnik : Glasilo Hrvatskog agronomskog društva
• Brojčani podaci: vol. 83, br. 3, str. 149-160
• p-ISSN: 0002-1954
• e-ISSN: 1848-8900
• DOI: https://doi.org/10.33128/ag.83.3.1
• URN:NBN: urn:nbn:hr:204:273275
• Datum objave publikacije: 2021
• URL dokumenta: https://hrcak.srce.hr/275612
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-02-28 08:15:51