Utjecaj metode ekstrakcije na sadržaj bioaktivnih spojeva stolisnika (Achillea millefolium L.)

master's thesis

• Title (english): The influence of the extraction method on the content of bioactive compounds of yarrow (Achillea millefolium L.)
• Author: Anamarija Novosel
• Mentor: Martina Grdiša (mentor)
• Committee member: Martina Grdiša (predsjednik povjerenstva)
• Committee member: Jana Šic Žlabur (član povjerenstva)
• Committee member: Filip Varga (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant biodiversity); Zagreb
• Defense date and country: 2024-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Stolisnik (Achillea millefolium L.) i njegovi pripravci (npr. ekstrakti) posjeduju široki spektar djelovanja koji se pripisuje bogatom sadržaju brojnih bioaktivnih spojeva i eteričnog ulja. Na sadržaj bioaktivnih spojeva ekstrakta utječu brojni čimbenici, a prvenstveno metoda ekstrakcije. U ovome radu prikupljeni biljni materijal podvrgnut je trima metodama ekstrakcije (maceracija, mikrovalna ekstrakcija, ultrazvučna ekstrakcija), a dobiveni ekstrakti analizirani su na sadržaj bioaktivnih spojeva te je utvrđen i njihov antioksidacijski kapacitet. Najveći sadržaj vitamina C (10,60 mg/100 g svježe tvari), ukupnih polifenola (495,29 mg GAE/100 g svježe tvari), flavonoida (271,95 mg GAE/100 g svježe tvari) i antioksidacijski kapacitet (FRAP metoda: 3994,41 μmol TE/L) zabilježen je kod ultrazvučno potpomognute ekstrakcije u trajanju od 10 minuta, dok je najveći sadržaj neflavonoida (224,68 mg GAE/100 g svježe tvari) zabilježen kod mikrovalno potpomognute ekstrakcije u trajanju od 10 minuta.

Abstract (english)

Yarrow (Achillea millefolium L.) and its medicinal preparations (e.g. extracts) have a broad spectrum of activity, which is attributed to the content of numerous bioactive compounds and essential oil. The content of the extract itself is influenced by numerous factors, as well as by the extraction method. In this work, the sampled plant material was subjected to three extraction methods (maceration, microwave-assisted extraction, ultrasound-assisted extraction), and the obtained extracts were analyzed for the content of bioactive compounds and their antioxidant capacity was determined. The highest content of vitamin C (10.60 mg/100 g dw), total polyphenols (495.29 mg GAE/100 g dw), flavonoids (271.95 mg GAE/100 g dw) and antioxidant capacity (FRAP method: 3994.41 μmol TE/L) was found in the ultrasound assisted extraction for 10 minutes, while the highest content of non-flavonoids (224.68 mg GAE/100 g dw) was recorded during microwave assisted extraction for 10 minutes.

• Keywords: antioksidacijski kapacitet; polifenoli; flavonoidi; mikrovalna ekstrakcija; ultrazvučna ekstrakcija
• Keywords (english): antioxidant capacity; polyphenols; flavonoids; microwave-assisted extraction; ultrasound-assisted extraction
• Language: croatian
• URN:NBN: urn:nbn:hr:204:506434
• Study programme: Title: Organic Agriculture with Agrotourism; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka ekološke poljoprivrede i agroturizma (magistar / magistra inženjer/inženjerka ekološke poljoprivrede i agroturizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-02 10:56:16