Simultana inkapsulacija srebrovih i cinkovih iona u biopolimerne mikročestice

master's thesis

• Title (english): Simultaneous encapsulation of silver and zinc ions into biopolymer microparticles
• Author: Ana Krčelić
• Mentor: Marko Vinceković (mentor)
• Committee member: Marko Vinceković (predsjednik povjerenstva)
• Committee member: Mirna Mrkonjić Fuka (član povjerenstva)
• Committee member: Luna Maslov Bandić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Chemistry); Zagreb
• Defense date and country: 2023-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Jedan od najvećih problema u svjetskoj komercijalnoj proizvodnji usjeva i hrane su
biljno patogeni bakterije i gljivice, što dramatično povećava troškove proizvodnje i smanjuje
produktivnost. Upotreba inovativnih agroekoloških formulacija jedan je od ključnih koraka u
zaštiti biljaka. Pripravljene su nove formulacije alginatnih mikročestica i mikrokapsula s Zn2+
ili istovremeno s Zn2+ i Ag+ i karakterizirane (veličina i morfologija mikročestica, učinkovitost
inkapsulacije, kapacitet inkapsulacije i bubrenje). Istraživanje molekulskih interakcija pomoću
FTIR spektroskopije otkrilo je složene interakcije između svih konstituenata mikročestica.
Karakteristike cinkovih i srebrovih kationa nisu nepovoljno utjecale na inkapsulaciju u
mikročesticama biopolimera, a njihova aktivnost ostala je prilično visoka.
In vitro profili otpuštanja cinkovih i srebrovih kationa analizirani su pomoću Korsmeyer-
Peppas empirijskog modela. Vrlo niske vrijednosti eksponenta n ukazale su da je Fickova
difuzija mehanizam koji kontrolira otpuštanje cinkovih i srebrovih iona. Niže vrijednosti
konstante otpuštanja k za mikrokapsule ukazuju na sporiju brzinu otpuštanja cinkovih i
srebrovih kationa u usporedbi s mikrosferama. Rezultati su pokazali da se pripravljene
mikročestice mogu potencijalno koristiti u zaštiti i prihrani biljaka.

Abstract (english)

One of the biggest problems to the world's commercial crop and food production are
plant pathogenic bacteria and fungi, which raises production costs dramatically and reduces
crop productivity. The use of innovative agroecological formulations is one of the most critical
steps in plant protection. Novel alginate microparticles (microspheres and microcapsules)
loaded with Zn2+ or simultaneously with Zn2+ and Ag+ were prepared and characterized
(microparticle size and surface morphology, encapsulation efficiency, loading capacity,
swelling). Investigation of molecular interactions in microparticles using FTIR spectroscopy
revealed complex interactions between all constituents. The zinc and silver cations
characteristics were not adversely affected by the encapsulation in biopolymer microparticles,
and their activity remained quite high.
The in vitro release profiles of Zn2+ and Ag+ ions were nalayzed by Korsmeyer–Peppas
empirical model. Very low values of the exponent n revealed Fickian diffusion was ratecontrolling
mechanism for both cations, Zn2+ and Ag+. Lower values of the release constant k
for microcapsules imply a slower release of Zn2+ or Ag+ ions than from microspheres. The
outcomes demonstrated that prepared microparticles can potentially be used in plant protection
and nutrition.

• Keywords: mikrokapsule; mikročestice; cinkovi ioni; srebrovi ioni
• Keywords (english): microspheres; microcapsules; zinc ions; silver ions
• Language: croatian
• URN:NBN: urn:nbn:hr:204:282377
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-15 08:03:33