Molekularne i imunološke metode u mikrobiologiji hrane

master's thesis

• Title (english): Molecular and immunological methods in food microbiology
• Author: Lucija Horvat
• Mentor: Nevijo Zdolec (mentor)
• Committee member: Nevijo Zdolec (predsjednik povjerenstva)
• Committee member: Lidija Kozačinski (član povjerenstva)
• Committee member: Mirna Mrkonjić Fuka (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2021-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Patogeni koji se prenose hranom mogu se pronaći u različitim namirnicama, a važno ih je otkriti kako bi se spriječile bolesti koje se prenose hranom i/ili vodom. Konvencionalne metode koje se koriste za otkrivanje patogena prenosivih hranom dugotrajne su i zahtijevaju puno rada. Stoga su razvijene različite tzv. brze metode za otkrivanje uzročnika bolesti koji se prenose hranom. Te metode mogu se kategorizirati u metode na bazi nukleinskih kiselina, na bazi biosenzora i imunološke metode. Ovaj pregled naglašava prednosti i nedostatke molekularnih i imunoloških metoda. Uključene metode detekcije su lančana reakcija polimerazom (PCR) i njene podvarijante, te enzimski imunoenzimski test (ELISA) i njegove podvarijante. Brze metode općenito su osjetljive, vremenski učinkovite, specifične i zahtijevaju manje rada. Razvoj ovih metoda važan je za prevenciju i liječenje bolesti koje se prenose hranom.

Abstract (english)

Foodborne pathogens can be found in a variety of foods, and it is important to detect them in order to prevent foodborne and/or waterborne diseases. Conventional methods used to detect foodborne pathogens are time consuming and require a lot of work. Therefore, various so-called rapid methods for detecting foodborne pathogens are developed. These methods can be categorized into nucleic acid-based, biosensor-based, and immunological methods. This review highlights the advantages and disadvantages of molecular and immunological methods. Included detection methods are polymerase chain reaction (PCR) and its subvariants, and enzyme-linked immunosorbent assay (ELISA) and its subvariants. Fast methods are generally sensitive, time-efficient, specific, and require less work. The development of these methods is important for the prevention and treatment of foodborne diseases.

• Keywords: molekularne metode; imunološke metode; PCR; ELISA
• Keywords (english): molecular methods; immunological methods; PCR; ELISA
• Language: croatian
• URN:NBN: urn:nbn:hr:204:161669
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2021-12-03 12:12:52