Uspješnost zakorjenjivanja jabuke sorte Gala ovisno o predtretmanu citokininima

master's thesis

• Title (english): The effect of cytokinin pretreatment on rooting success of Gala apple variety
• Author: Matea Jurić
• Mentor: Anita Bošnjak Mihovilović (mentor)
• Committee member: Anita Bošnjak Mihovilović (predsjednik povjerenstva)
• Committee member: Snježana Kereša (član povjerenstva)
• Committee member: Ivanka Habuš Jerčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Breeding Genetics, Biometrics and Experimentation); Zagreb
• Defense date and country: 2021-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Kroz ovaj rad ispitan je utjecaj citokinina na uspješnost zakorjenjivanja jabuke sorte Gala ovisno o predtretmanu citokininima. Upotreba citokinina nužna je u fazi multiplikacije izdanaka. Međutim rezidui citokinina mogu negativno utjecati na zakorjenjivanje izdanaka. S obzirom da je u dostupnoj literaturi poznato da se prirodni citokinin meta-topolin (mT) brže razgrađuje u biljnom tkivu u odnosu na češće korišten 6-benzilaminopurin (BAP) i ima manje negativnih utjecaja na razvoj korijenja i zakorjenjivanje, ovim istraživanjem pokazalo se suprotnim. Jedan od glavnih razloga velike primjene BAP-a u mikropropagaciji je zbog toga što je stabilniji u odnosu na ostale citokinine, teže oksidira uz pomoć svjetlosti i jeftiniji je. Zbog različitih nuspojava prilikom korištenja kao što su nekroza vrha izdanka, inhibicija zakorjenjivanja te teškoće prilikom aklimatizacije kao alternativna zamjena u literature se navodi meta-topolin.
U cilju procjene post-efekta citokinina na zakorjenjivanje jabuke sorte Gala, u ovom istraživanju izdanci jabuke sorte Gala su multiplicirani u in vitro uvjetima na medijima s dva različita regulatora rasta. Korišteni su citokinini BAP i mT s pet različitih koncentracija svaki. Nakon postignutog dovoljnog broja eksplantata na hranjivom mediju za multiplikaciju, izdanci jabuke postavljeni su na medij za zakorjenjivanje s auksinom Indol-3-maslačnom kiselinom (IBA) i na kontrolni medij. Izdanci razvijeni na podlozi sa mT bili su duži u odnosu na izdanke koji su razvijeni na podlozi sa BAP-om, ali su oni imali veći broj multipliciranih izdanaka u odnosu na mT. Nakon što su se izdanci zakorjenili procjenjena je uspješnost zakorjenjivanja s obzirom na prethodni medij gdje su korištene različite koncentracije BAP-a i mT-a. Zakorjenjivanje je bilo uspješnije na podlogama s dodatkom BAP-a kao i postotak aklimatiziranih biljaka.

Abstract (english)

In this work, the influence of cytokinins on rooting success of Gala cultivar apple in dependence on pretreatment with cytokinins was studied. The use of cytokinins is necessary in the shoot multiplication phase. However, cytokinin residues may negatively affect shoot rooting. As it is known from the available literature that the natural cytokinin meta-topoline (mT) is degraded faster in plant tissues compared to the more commonly used 6-benzylaminopurine (BAP) and will have fewer negative effects on root development and rooting this study proved the opposite. One of the main reasons for the large application of BAP in micropropagation is that it is more stable compared to other cytokinins, it oxidizes more difficult with the help of light and it is cheaper and easier to obtain. Due to various side effects during use such as shoot tip necrosis, rooting inhibition and difficulty in acclimatization, meta-topoline is cited as an alternative in the literature.
In n this study to detect post-effect of cytokinins on rooting of Gala variety apple, shoots of Gala apples were first multiplied on medium with two different growth regulators. Cytokines BAP and mT were used at five different concentrations. After a sufficient number of explants were obtained on the nutrient medium for multiplication, apple shoots were placed on the rooting medium with auxin Indole-3-butyric acid (IBA) and on the control medium. The shoots were rooted on MS medium and the rooting success was evaluated with respect to the previous medium where different concentrations of BAP and mT were used.
Shoots that developed on media with addition of mT were much longer compared to shoots developed on media with BAP. However, higher number of multiplied shoots were developed on media containing BAP. Rooting as well as success of acclimatization were more successful in plants developed on media with the addition of BAP.

• Keywords: citokinini; multiplikacija; zakorjenjivanje; aklimatizacija; 6-benzilaminopurin; meta-topolin
• Keywords (english): cytokinins; multiplication; rooting; acclimatization; 6-benzylaminopurine; meta-topoline
• Language: croatian
• URN:NBN: urn:nbn:hr:204:889946
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-17 11:29:57