Polimorfizam DGAT1 gena u subpopulaciji istočnofrizijske ovce, safolka i travničke pramenke u Republici Hrvatskoj

master's thesis

• Title (english): Polymorphism of DGAT1 gene in subpopulation of East Friesian sheep, Suffolk and Travnik pramenka sheep in the Republic of Croatia
• Author: Marina Šalković
• Mentor: Ante Kasap (mentor)
• Committee member: Ante Kasap (predsjednik povjerenstva)
• Committee member: Jelena Ramljak (član povjerenstva)
• Committee member: Krešimir Salajpal (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Animal Science and Technology); Zagreb
• Defense date and country: 2020-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Enzim diacilglicerol O-aciltransferaza (DGAT1) ima važnu ulogu u sintezi triglicerida, a gen koji ga kodira predstavlja kandidatni gen za mnoga svojstva kakvoće i količine ovčjeg mesa i mlijeka. Cilj istraživanja bio je utvrditi polimorfizam DGAT1 gena u subpopulacijama istočnofrizijske ovce, safolka i travničke pramenke u Hrvatskoj. Također, cilj je bio razmotriti potencijal selekcije ovaca kod istraživanih pasmina temeljem dobivenih rezultata i publiciranih učinaka gena DGAT1 na neka važna svojstva u proizvodnji mesa i mlijeka. Istraživani lokus je bio polimorfan kod safolka i travničke pramenke, a monomorfan kod istočnofrizijske ovce. Izrazita prevalencija alela C i genotipa CC utvrđena je kod sve tri pasmine. Očekivana heterozigotnost kod safolka (He=0,073) i travničke pramenke (He=0,127) je bila izrazito niska dok je kod istočnofrizijske ovce bila ravna nuli (He=0,0). Temeljem razlika između opaženih i očekivanih frekvencija genotipova, utvrđeno je da su istraživane populacije u Hardy-Weinbergovoj ravnoteži (P>0,05). Uzimajući u obzir utvrđene frekvencije genotipova i poznate (publicirane) utjecaje polimorfizma DGAT1 gena na veći broj svojstava, može se zaključiti da je profil DGAT1 u istraživanim populacijama u povoljan za količinu proizvedenog mesa i kvalitetu mlijeka, a nepovoljan za količinu mlijeka i kvalitetu mesa

Abstract (english)

Enzyme diacylglycerol O-acyltransferase (DGAT1) plays an important role in the final stage of triglyceride synthesis. The gene that encodes this enzyme represents an important candidate gene for numerous quantitative and qualitative meat and dairy traits. The study aimed to determine the polymorphism of this gene in subpopulations of the East Friesian, Suffolk and Travnik pramenka breed in Croatia. The study also aimed to roughly examine the potential of selection in these populations, based upon the obtained results and known (published) effects of the gene on some important meat and dairy traits. The examined locus was polymorphic in Suffolk and Travnik pramenka, while it was monomorphic in the East Friesian breed. Allele C and genotype CC were prevalent in all of the examined breeds. Extremely low expected heterozygosity was found in Suffolk (He=0.073), and Travnik pramenka (He=0.127), while it equated zero in the East Friesian breed (He=0). Based upon the differences between the determined and expected frequencies of the genotypes, it was determined that all the populations under consideration were in Hardy-Weinberg equilibrium (P>0.05). By taking into account the determined frequencies of genotypes and the known (published) effects of DGAT1 polymorphism on numerous traits, it can be concluded that the DGAT1 profile in the studied populations is favorable for meat yield and milk quality traits and unfavorable for milk quantity and meat quality traits.

• Keywords: gen DGAT1; ovce; mast; meso; mlijeko
• Keywords (english): DGAT1 gene; sheep; fat; meat; milk
• Language: croatian
• URN:NBN: urn:nbn:hr:204:874181
• Study programme: Title: Production and Processing of Meat; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka proizvodnje i prerade mesa (magistar / magistra inženjer/inženjerka proizvodnje i prerade mesa)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-29 12:14:17