Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)

master's thesis

• Title (english): Influence of age and gender on the biochemical properties of the honey bee hemolymph (Apis mellifera L.)
• Author: Jelena Horvatinec
• Mentor: Lidija Svečnjak (mentor)
• Committee member: Lidija Svečnjak (predsjednik povjerenstva)
• Committee member: Dragan Bubalo (član povjerenstva)
• Committee member: Marko Vinceković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Fisheries, Beekeeping, Game Management and Special Zoology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Hemolimfa je tekućina otvorenog sustava cirkulacije medonosne pčele (Apis mellifera
L.) koja predstavlja funkcionalni ekvivalent krvi i limfe sisavaca. Sloţenog je kemijskog
sastava te uz šećere i lipidne komponente, sadrţi preko 300 različitih proteina. Dosadašnjim
su istraţivanjima utvrĎene odreĎene razlike u sastavu hemolimfe izmeĎu kasti pčela, različitih
razvojnih stadija te dobnih skupina pčela. MeĎutim, detaljna kemijska karakterizacija sastava
hemolimfe nije provedena. Stoga je cilj ovog rada bio utvrditi biokemijske značajke i razlike
u sastavu hemolimfe pčela u ovisnosti o dobi i spolu temeljem kemijskog fingerprintinga
hemolimfe. Istraţivanje je provedeno na 3 pokusne pčelinje zajednice. Ekstrakcijom iz
područja prsišta iz svake su pokusne zajednice prikupljeni uzorci hemolimfe pet dobnih
skupina radilica (1, 7, 14, 24 i 42 dana; n=338) i trutova (n=8) te su analizirani metodom
FTIR-ATR spektroskopije. Rezultati istraţivanja pokazali su kako hemolimfa pčela daje
karakterističan IR spektar, a temeljem spektralnih varijacija utvrĎeno je kako se hemolimfa
pčela ovisno o dobi i spolu ne razlikuje kvalitativno, već postoje razlike u udjelu pojedinih
komponenata sastava.

Abstract (english)

Hemolymph is a liquid of the open circulation system of the honey bee (Apis mellifera L.),
which representes a functional equivalent of mammalian blood and lymph. It has a complex
chemical composition and contains over 300 different proteins along with sugars and lipid
components. Previous studies have revealed certain differences in the hemolymph
composition between the honey bee castes, different developmental stages and age groups of
bees. However, a detailed chemical characterization of the hemolymph composition has not
been performed. Therefore, the aim of this study was to determine the biochemical
characteristics and differences in honey bee hemolymph composition depending on the age
and gender based on the hemolymph chemical fingerprinting. The study was conducted on 3
experimental honey bee colonies. Hemolymph samples of five age groups of workers (1, 7,
14, 24 and 42 days old; n = 338) and drones (n=8) were collected by thorax extraction from
each experimental colony, and analysed by FTIR-ATR spectroscopy. The results showed that
honey bee hemolymph provides a characteristic IR spectrum, and based on spectral variations
it was determined that the hemolymph of the honey bee does not differ qualitatively
depending on age and gender, but there are differences in the proportion of individual
compositional components.

• Keywords: medonosna pčela; hemolimfa; FTIR-ATR spektroskopija; kemijska karakterizacija; dobne i spolne razlike
• Keywords (english): honey bee; hemolymph; FTIR- ATR spectroscopy; chemical characterization; age and gender differences
• Language: croatian
• URN:NBN: urn:nbn:hr:204:588441
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-11-07 13:37:09