Abstract | Suvremena istraživanja pokazuju da se različiti proizvodni rezultati životinja hranjenih žitaricama ne mogu objasniti samo razlikama u sadržaju i probavljivosti njihovih hranjivih tvari te da se uzimanjem u obzir i kinetike probavljivosti povisuje preciznost procjene hranjivosti. Silaža zrna kukuruza je standardno krmivo u tovu svinja, a u dostupnoj literaturi nema podataka o kinetici probavljivosti njenog škroba u svinja. Nadalje, nema podataka o utjecaju dužine stajanja silaže na promjene kinetike razgradnje škroba naših hibrida kukruza. Cilj ovog istraživanja bio je određivanje promjena kinetike in vitro ilealne razgradnje škroba visoko-vlažnog zrna kukuruza. Spontano siliranje zrna kukuruza hibrida Bc 344 provelo se u vakuum vrećicama, a uzorkovanja su provedena otvaranjem vrećica koje predstavljaju 0., 15., 48., 98., 182., 274. i 364. dan stajanja. In vitro procedura probavljivosti provela se prema kinetici prvog reda koja prati probavu u želucu i tankom crijevu svinja. Doseg razgradnje škroba pratio se nakon 0,25, 0,50, 0,75, 1, 2, 3, 4, 5 i 6 sati, a za izračun brzine u svim uzorcima određen je sadržaj škroba i šećera. S vremenom stajanja silaže dolazi do opadanja sadržaja šećera, dok su varijacije u sadržaju škroba unutar 3%. Probavljivost suhe tvari linearno raste sa stajanjem silaže od 80,84 do 88,26%. U prvih sat vremena inkubacije razgradilo se 62,74% škroba, dok se sav škrob u silažama koje su stajale više od 15 dana razgradi do četiri sata. Brzina probavljivosti škroba raste sa stajanjem silaže visoko-vlažnog zrna kukuruza što se može opisati linearnom i kvadratnom jednadžbom ovisnosti. |
Abstract (english) | Recent studies show that differences in production performance of animals fed with cereals could not be explained only with differences in nutrient content and digestibility; when digestibility kinetics is taken into account, the precision of nutritional evaluation of feeds is considerably improved. Although high moisture corn silage is widely used feed in pig fattening, there is no data about its starch digestibility kinetics in the available literature. Furthermore, there is no data regarding the effect of storage time on changes in starch digestibility kinetics of Croatian hybrids. The aim of this research was to determine changes in in vitro ileal digestibility of high moisture corn silage. Spontaneous fermentation of hybrid Bc 344 grain was performed in vacuum bags, while sampling was performed by the opening of bags representing 0, 15, 48, 98, 182, 274 and 364 days of storage. In vitro digestibility procedure was done according to the first order kinetics mimicking digestion in stomach and small intestine. The extent of starch digestion was determined after 0,25, 0,5, 0,75, 1, 2, 3, 4, 5 and 6 hours of incubation and in all samples free sugar and total starch contents were determined for digestibility rate calculations. Free sugar content decreases with increasing storage time while variations in starch content were within 3%. Dry matter digestibility linearly increased from 80,84 to 88,26% with increasing storage time. A 62,74% of starch was digested in the first hour of digestion, and after 4 hours of digestion all starch was digested in samples stored more than 15 days. Starch digestibility rate increased with increasing storage time of high moisture corn silage which can be described with linear and quadratic equations. |