Analiza sastava i sadržaja piretrina u samoniklim populacijama dalmatinskog buhača (Tanacetum cinerariifolium)

master's thesis

• Title (english): Analysis of the composition and content of pyrethrins in Dalmatian pyrethrum (Tanacetum cinerariifolium) natural populations
• Author: Laura Gradinšćak
• Mentor: Martina Grdiša (mentor)
• Committee member: Martina Grdiša (predsjednik povjerenstva)
• Committee member: Jana Šic Žlabur (član povjerenstva)
• Committee member: Klaudija Carović-Stanko (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant biodiversity); Zagreb
• Defense date and country: 2023-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Piretrin je insekticidni spoj dalmatinskog buhača. Smjesa je šest aktivnih sastavnica; piretrina I i II, cinerina I i II te jasmolina I i II. Piretrin I i II su najzastupljenije sastavnice i njihov omjer određuje insekticidni potencijal piretrinskog ekstrakta. Istraživanje sastava i sadržaja piretrina provedeno je na sedam samoniklih populacija dalmatinskog buhača (P01 Žman, Dugi otok, P02 Sevid, P03 Budva, P04 Srđ, P05 Vis, P06 Korčula, P07 Murter). Ultrazvučna ekstrakcija korištenjem acetona kao ekstrakcijskog otapala korištena je za izdvajanje piretrina iz osušenih cvatnih glavica. Dobiveni ekstrakti piretrina analizirani su tekućinskom kromatografijom visoke djelotvornosti (HPLC DAD-UV/VIS). Varijabilnost u sastavu i sadržaju piretrina između prirodnih populacija utvrđene su analizom varijance (ANOVA) i analizom glavnih sastavnica (PCA). Dobiveni rezultati ukazuju na široku kemijsku varijabilnost prirodnih populacija dalmatinskog buhača. Prosječan sadržaj ukupnih piretrina u masi suhog cvata kretao se od 0,91 % (P02 Sevid) do 1,27 % (P03 Budva), a prosječni omjer piretrina I i piretrina II od 1,08 (P02 Sevid) do 1,74 (P04 Srđ).

Abstract (english)

Pyrethrin is an insecticidal compound of Dalmatian pyrethrum. The mixture consists of six active components: pyrethrin I and II, cinerin I and II and jasmolin I and II. Pyrethrin I and II are the most abundant components and their ratio determines the insecticidal potential of the pyrethrin extract. Studies on the composition and content of pyrethrins were conducted on seven wild populations of Dalmatian pyrethrum (P01 Žman, Dugi otok, P02 Sevid, P03 Budva, P04 Srđ, P05 Vis, P06 Korčula, P07 Murter). Ultrasonic extraction with acetone as extraction solvent was performed to isolate pyrethrins from the dried flower heads. The obtained pyrethrin extracts were analyzed by high-performance liquid chromatography (HPLC DAD-UV /VIS). Variability in composition and content of pyrethrins between natural populations was determined by analysis of variance (ANOVA) and principal component analysis (PCA). The obtained results indicate a great chemical variability among natural populations of Dalmatian pyrethrum. The average content of total pyrethrins in flower dry matter ranged from 0.91% (P02 Sevid) to 1.27% (P03 Budva), and the average ratio of pyrethrin I to pyrethrin II ranged from 1.08 (P02 Sevid) to 1.74 ( P04 Srđ).

• Keywords: autohtona vrsta; Hrvatska; prirodni insekticid; endem; ekološka poljoprivreda
• Keywords (english): indigenous species; Croatia; natural insecticide; endemic; organic agriculture
• Language: croatian
• URN:NBN: urn:nbn:hr:204:196640
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-15 09:28:02