In vitro metode ozdravljenja tradicijskog kultivara krumpira 'Brinjak' od M-virusa krumpira

master's thesis

• Title (english): In vitro methods of elimination of potato virus M from traditional potato cultivar ‘Brinjak’
• Author: Valentina Rohrbacher
• Mentor: Snježana Kereša (mentor)
• Committee member: Snježana Kereša (predsjednik povjerenstva)
• Committee member: Milan Pospišil (član povjerenstva)
• Committee member: Darko Vončina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Breeding Genetics, Biometrics and Experimentation); Zagreb
• Defense date and country: 2023-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

'Brinjak' je tradicijski kultivar krumpira koji se generacijama uzgaja u Lici. Otporan je na plamenjaču i običnu krastavost krumpira, no zbog zaraze virusima u proizvodnji ne može postići svoj puni potencijal rodnosti. M-virus krumpira (eng. potato virus M, PVM) može smanjiti prinos gomolja od 11-45%. Primjenjujući tri različite in vitro metode pokušali smo eliminirati M-virus krumpira kojim je ovaj kultivar zaražen. Korištene su metode kulture izoliranih meristema bez prethodne terapije, kulture meristema nakon termoterapije na 37 C i kulture meristema nakon elektroterapije. Metodom ELISA ispitana je prisutnost M-virusa krumpira u listovima krumpira 'Brinjak' tretiranih trima navedenim metodama. Rezultati istraživanja su pokazali da je najveći postotak biljaka oslobođenih od M-virusa krumpira dobiven nakon tretmana termoterapijom u kombinaciji s kulturom meristema (94,1 %). Elektroterapijom u kombinaciji s kulturom meristema oslobođeno je 64,7 % uzoraka krumpira, a izolacijom meristema bez prethodnih tretmana 53,8 % prethodno zaraženih uzoraka kultivara 'Brinjak'.

Abstract (english)

‘Brinjak’ is a traditional potato cultivar that has been grown in Lika for generations. It is resistant to late blight and common potato scab. However, due to virus infection during production, it cannot achieve its full yield potential. PVM (Potato virus M) can reduce tuber yield by 11-45%. Applying three different in vitro methods, we tried to eliminate the PVM with which this cultivar was infected. The methods that were used were isolated meristems without previous therapy, meristems after thermotherapy at 37 C and meristems after electrotherapy. Then, the presence of PVM virus, in the leaves of cultivar ‘Brinjak’, was determined by using the ELISA method. The results showed that the highest percentage of virus-free plants of potato ‘Brinjak’ was obtained after treatment with thermotherapy in combination with meristem culture (94.1%). Electrotherapy in combination with meristem culture eliminated viruses from 64.7% of treated plants, while by the culture of isolated meristems without prior treatment PVM was eliminated from 53.8% of the plants.

• Keywords: Brinjak; PVM; ELISA; in vitro; termoterapija; elektroterapija; kultura meristema
• Keywords (english): Brinjak; PVM; ELISA; in vitro; thermotherapy; electrotherapy; meristem culture
• Language: croatian
• URN:NBN: urn:nbn:hr:204:123002
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-09-25 08:34:46