Potencijal pčelinjeg otrova (Apis mellifera L.) u inhibiciji rasta multirezistentnih sojeva Klebsiella pneumoniae

master's thesis

• Title (english): Potential of bee venom (Apis mellifera L.) in growth inhibition of multiresistant strains of Klebsiella pneumoniae
• Author: Anja Benc
• Mentor: Mirna Mrkonjić Fuka (mentor)
• Committee member: Mirna Mrkonjić Fuka (predsjednik povjerenstva)
• Committee member: Lidija Svečnjak (član povjerenstva)
• Committee member: Marko Vinceković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2022-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Rastuća prevalencija Klebsiella pneumoniae otpornih na karbapeneme i kolistin postaje sve veći problem na globalnoj razini, s obzirom da se navedeni antibiotici koriste kao lijekovi zadnje linije obrane u liječenju infekcija uzrokovanih ovim bakterijama. Kationski antimikrobni peptidi (CAP) prirodni su spojevi s dokazanom antimikrobnom aktivnošću, a jedan od značajnih izvora CAP-a je pčelinji otrov (apitoksin) kojeg proizvode medonosne pčele (Apis mellifera L.). Cilj ovog rada jest odrediti utjecaj pčelinjeg otrova na karbapenem rezistentne sojeve K. pneumoniae izolirane iz otpadnih voda. Analizom obrazaca dobivenih rep-PCR metodom selektirani su reprezentativni sojevi K. pneumoniae. Određene su minimalne inhibitorne (MIK) i minimalne baktericidne (MBK) koncentracije metodom serijske mikrodilucije na Mueller-Hinton podlozi. MIK vrijednost kretala se u rasponu od 25-100 μg/mL dok je MBK bila u rasponu od 25-200 μg/mL. Najčešće su MIK vrijednosti bile jednake MBK vrijednostima.

Abstract (english)

The growing prevalence of Klebsiella pneumoniae resistant to carbapenems and colistin is becoming a growing problem at the global level, considering that these antibiotics are used as the last line of defense in the treatment of infections caused by these bacteria. For this reason, the demand for alternative antimicrobial compounds that will effectively inhibit the growth of these enterobacteria is growing. Cationic antimicrobial peptides (CAP) are natural compounds with proven antimicrobial activity, and one of the significant sources of CAP is a bee venom (apitoxin) produced by the honey bees (Apis mellifera L.). The aim of this study is to determine the influence of bee venom on carbapenem resistant strains of Klebsiella pneumoniae isolated from wastewater. By analyzing the fingerprints obtained by the rep-PCR method, representative strains of K. pneumoniae were selected. Minimal inhibitory (MIK) and minimal bactericidal (MBK) concentrations were determined by the method of serial microdilulation on the Mueller-Hinton substrate. The MIC value was in the range of 25-100 μg/mL, while the MBK was in the range of 25-200 μg/mL. Most often, MIK values were equal to MBK values.

• Keywords: pčelinji otrov; Klebsiella pneumoniae; rep- PCR; minimalna inhibitorna koncentracija (MIK); minimalna baktericidna koncentracija (MBK)
• Keywords (english): bee venom; Klebsiella pneumoniae; rep-PCR; minimum inhibitory concentration (MIC); minimum bactericidal concentration (MBC)
• Language: croatian
• URN:NBN: urn:nbn:hr:204:797569
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-10-13
• Created on: 2022-10-13 12:18:25