Utjecaj matične mliječi na višestruko rezistentne patogene gram negativne bakterije

master's thesis

• Title (english): The influence of royal jelly on multiresistant gram negativepathogenic bacteria
• Author: Monika Kosec
• Mentor: Mirna Mrkonjić Fuka (mentor)
• Committee member: Mirna Mrkonjić Fuka (predsjednik povjerenstva)
• Committee member: Lidija Svečnjak (član povjerenstva)
• Committee member: Marko Vinceković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Microbiology); Zagreb
• Defense date and country: 2022-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Cilj ovog rada je ispitati djelovanje matične mliječi prikupljene na području Republike Hrvatske prema višestruko rezistentnim sojevima Klebsiella pneumoniae i Acinetobacter baumannii izoliranim iz otpadnih voda. Metodom difuzije bunara u agaru kvalitativno je određena antibakterijska aktivnost matičnih mliječi te su mikrodilucijskom metodom određene minimalne inhibitorne (MIK) i minimalne baktericidne (MBK) koncentracije. Svi analizirani uzorci matičnih mliječi djeluju inhibitorno i baktericidno na sve ispitane sojeve, ali u različitim koncentracijama. Zone inhibicije rasta pokazuju da su sojevi K. pneumoniae otporniji na djelovanje matične mliječi od A. baumannii (K. pneumoniae: 11,8 mm ± 2,8 mm; A. baumannii 14,4 mm ± 3,8 mm, ANOVA, Bonferroni test, p<0,001). Koncentracija matične mliječi od 27,01 mg/mL ± 15,80 mg/mL je djelovala baktericidno na sojeve A. baumannii, dok je za sojeve K. pneumoniae bila potrebna koncentracija od 120,44 mg/mL ± 98,34 mg/mL.

Abstract (english)

The aim of this work is to examine the action of royal jelly collected in the Republic of Croatia against multi-resistant strains of Klebsiella pneumoniae and Acinetobacter baumannii isolated from wastewater. The antibacterial activity of the royal jelly was qualitatively determined using the well diffusion method in agar, and the minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations were determined using the microdilution method. All analyzed samples of royal jelly have an inhibitory and bactericidal effect on all tested strains, only in different concentrations. Zones of growth inhibition show that K. pneumoniae strains are more resistant to the action of royal jelly than A. baumannii (K. pneumoniae: 11,8 mm ± 2,8 mm; A. baumannii 14,4 mm ± 3,8 mm, ANOVA, Bonferroni test, p<0.001). Concentration of royal jelly of 27,01 mg/mL ± 15,80 mg/mL had a bactericidal effect on A. baumannii strains, while K. pneumoniae strains required a concentration of 120,44 mg/mL ± 98,34 mg/mL.

• Keywords: matična mliječ; antibakterijska aktivnost; Klebsiella pneumoniae; Acinetobacter baumannii; minimalna inhibitorna (MIK) i minimalna baktericidna koncentracija (MBK)
• Keywords (english): royal jelly; antibacterial activity; Klebsiella pneumoniae; Acinetobacter baumannii; minimum inhibitory (MIC) and minimum bactericidal concentration (MBC)
• Language: croatian
• URN:NBN: urn:nbn:hr:204:500486
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-30
• Created on: 2022-09-30 07:00:02