Optimizacija medija za predkulturu mikroreznica in vitro u postupku krioprezervacije sorte vinove loze 'Graševina'

master's thesis

• Title (english): Optimization of preculture medium for in vitro microcuttings in the procedure of cryopreservation of the grapevine cultivar 'Graševina'
• Author: Paulina Piškor
• Mentor: Zvjezdana Marković (mentor)
• Committee member: Zvjezdana Marković (predsjednik povjerenstva)
• Committee member: Domagoj Stupić (član povjerenstva)
• Committee member: Željko Andabaka (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Viticulture and Enology); Zagreb
• Defense date and country: 2021-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Vinova loza (Vitis vinifera L.) je jedna od najstarijih poljoprivrednih kultura. Široka primjena u gospodarstvu čini je jednom od ekonomski najvažnijih poljoprivrednih voćnih kultura u svijetu. Republika Hrvatska je značajan gen centar za domaće, ali i strane sorte pa je cilj vinogradarske prakse očuvati i revitalizirati njihov uzgoj. Krioprezervacija je najbolji i najefikasniji način čuvanja genetskog materijala. To je proces u kojem se biljno tkivo izlaže niskim temperaturama tekućeg dušika te se blokiraju metaboličke funkcije biljnog materijala. U procesu krioprezervacije različite predkulture mikroreznica, krioprotektivna sredstva i koraci pri smrzavanju mogu biti toksični i izazvati stres kod sorata vinove loze.
Cilj ovog rada bio je optimatizirati medij za predkulturu mikroreznica dodatkom antioksidansa (salicilne kiseline) u svrhu postizanja uspješne regeneracije vegetacijskih vršaka sorte 'Graševine'. Istraživanje je provedeno na mikroreznicama sorte graševine, posađene na ½ MS medij, sa ili bez dodatka citokinina benzilaminopurina te različitim koncentracijama salicilne kiseline (0, 0.1, 0.5 i 1 mMol). Najveći postotak regeneriranih biljaka bio je na mediju bez dodatka salicilne kiseline i BAP-a (68,38%), a najmanji na mediju sa dodatkom salicilne kiseline koncentracije 0.1 mMol i dodatkom 1μmol BAP-a (35,00%). Nakon provedenog postupka krioprezervacije sorte 'Graševine' veći uspjeh regeneracije pokazali su kontrolni eksplantati koji nisu bili podvrgnuti smrzavanju, u odnosu na smrznute eksplantate. Dobiveni rezultati upućuju da su potrebna dodatna istraživanja za uspješnu krioprezervaciju ove sorte.

Abstract (english)

Grapevine (Vitis vinifera L.) is one of the oldest agricultural culture. Wide application of this culture in the economy makes it one of the most important agricultural fruit culture in the world. Republic of Croatia is important gene center for native, as well as for introduced cultivars of grapevine, so the viticulture's aim is to conserve and revitalize its cultivation. Crypreservation is the most efficient procedure of the conservation of the plant material. In the procedure different preculture of microcuttings, cryoprotectants and steps during freezing can be toxic and make the stress within cultivars of grapevine.
The aim of this study is the optimization of preculture medium for preculture of microcuttings with addition of antioksidans (salicylic acid) with the purpose of the successful growth of shoot tips of the cultivar 'Graševina'. The study was made on microcuttings of cultivar 'Graševina', planted on ½ MS medium, with or without cytokinins benzylaminopurine and different concentrations of salicylic acids (0, 0.1, 0.5 and 1 mMol). The highest percentage of regenerated plants was achieved on the medium without the salicylic acid and BAP (68, 38%), and the lowest on the medium with addition of salicylic acid in concentration 0.1 mMol and supplement of 1μmol BAP (35, 00%). After cryopreservation, of microcuttings of the cultivar 'Graševina', the higher results of regeneration was achieved in controled explants in comparioson on the freezing ones. Given results are implying that some additional studies should be done for successful crzopreservation of this cultivar.

• Keywords: vinova loza; mikroreznice in vitro; salicilna kiselina; krioprezervacija
• Keywords (english): grapevine; microcuttings in vitro; salicylic acids; cryopreservation
• Language: croatian
• URN:NBN: urn:nbn:hr:204:260233
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2021-11-24 12:36:39