Otpornost na cefalosporine treće generacije u enterobakterija iz otpadnih voda

master's thesis

• Title (english): Resistance to third generation cephalosporins in enterobacteria from wastewater
• Author: Marija Parać
• Mentor: Mirna Mrkonjić Fuka (mentor)
• Committee member: Mirna Mrkonjić Fuka (predsjednik povjerenstva)
• Committee member: Marko Vinceković (član povjerenstva)
• Committee member: Vilim Filipović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2021-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Cefalosporini treće generacije spadaju među najčešće korištene antibiotike za tretiranje raznih infekcija kojima su uzročnici enterobakterije. Njihovo masovno korištenje potaknulo je razvoj otpornosti kod enterobakterija koje se sve češće pronalaze u otpadnim vodama. Cilj ovoga rada bio je odrediti prisutnost gena za otpornost na cefalosporine treće generacije, poglavito gena koji kodiraju β-laktamaze proširenog spektra djelovanja (ESBL) u enterobakterija iz zagrebačkih komunalnih i bolničkih otpadnih voda te utvrditi specifične genske varijante. U analiziranih 104 izolata detektirana je prisutnost različitih ESBL gena (blaCTX-M, blaTEM, blaSHV, blaGES) primjenom lančane reakcije polimerazom (PCR) i agarozne gel elektroforeze. Sekvenciranjem umnoženih gena utvrđena je prisutnost 12 genskih varijanti. Većina izolata (85,58%) posjedovala je 2 ili više ESBL gena, dok je manji udio izolata (16,35%) posjedovao 3 ili više ESBL gena uz pojavu višestruko otpornog fenotipa. Rezultati ovog istraživanja ukazuju da su bolničke i obrađene komunalne otpadne vode rezervoari ESBL-producirajućih enterobakterija, često višestruko otpornih, što predstavlja rizik za daljnje širenje tih bakterija u okoliš i potencijalni prijenos na ljude.

Abstract (english)

Third-generation cephalosporins are among the most commonly used antibiotics to treat various infections caused by enterobacteria. Their widespread use has induced the development of resistance in enterobacteria, which are increasingly found in wastewater. The aim of this study was to determine the presence of third generation cephalosporin resistance genes, especially genes encoding extended spectrum β-lactamases (ESBL) in enterobacteria from Zagreb municipal and hospital wastewater and to identify specific gene variants. In the analyzed 104 isolates, the presence of various ESBL genes (blaCTX-M, blaTEM, blaSHV, blaGES) was detected using polymerase chain reaction (PCR) and agarose gel electrophoresis. Sequencing of amplified genes revealed the presence of 12 gene variants. Most isolates (85.58%) possessed 2 or more ESBL genes, while a smaller proportion of isolates (16.35%) possessed 3 or more ESBL genes with the appearance of a multidrug-resistant phenotype. The results of this study indicate that hospital and treated municipal wastewater are reservoirs of ESBL-producing enterobacteria, often multi-resistant, posing a risk for further spread of these bacteria to the environment and potential transmission to humans.

• Keywords: enterobakterije; ESBL; cefalosporini treće generacije; PCR; antibiotička otpornost; višestruka otpornost; otpadne vode
• Keywords (english): enterobacteria; ESBL; third-generation cephalosporins; PCR; antibiotic resistance; multidrug-resistance; wastewater
• Language: croatian
• URN:NBN: urn:nbn:hr:204:876139
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2021-11-24 11:45:31