Genetska struktura tradicionalnih sorata glavatog kupusa (Brassica oleracea L. var. capitata f. alba)

master's thesis

• Title (english): Genetic structure of traditional varieties of head cabbage (Brassica oleracea L. var. capitata f. alba)
• Author: Josip Ćurak
• Mentor: Snježana Bolarić (mentor)
• Committee member: Snježana Bolarić (predsjednik povjerenstva)
• Committee member: Aleš Vokurka (član povjerenstva)
• Committee member: Ivanka Žutić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Breeding Genetics, Biometrics and Experimentation); Zagreb
• Defense date and country: 2021-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Tradicionalne sorte glavatog kupusa od velikog su gospodarskog i kulturnog značaja. Analizirane su četiri udomaćene (´Braunschweiger´, ´Kopenhaški´, ´Ditmar´, ´Slava´) i pet domaćih sorata (´Čepinski´, ´Varaždinski´, ´Ogulinski´, ´Žminjski´, ´Brgujski´). Varijabilnost germplazme je proučavana pomoću AFLP molekularnih markera da bi mogli utvrditi varijabilnost unutar sorata i između 9 sorata. Utvrđivanje varijabilnosti je cilj ovog istraživanja. Kako bi utvrdili varijabilnost koristili smo molekularne rezultate za analizirane sorte, te usporedili i napravili procjenu varijabilnosti na molekularnoj razini. Sjetva sjemena je obavljena početkom listopada 2019., a sjeme je sijano u kontejnere i čuvano u kontroliranim uvjetima. Od 9 proučavanih sorata analizirano je unutar svake sorte po 20 individua, a sveukupno 180 individua (9 sorti x 20 individua). U AFLP analizi su korištene četiri kombinacije početnica. Primarno je bilo potrebno napraviti izolaciju DNA, a kasnije je to pomoglo u analizi genetske strukture za koju su korišteni AFLP markeri. Matrica različitosti korištena je u analizi molekularne varijance (AMOVA), analizi udaljenosti između sorata i u STRUKTURE analizi.

Abstract (english)

Traditional varieties of head cabbage are of great economic and cultural importance. Four domesticated varieties (´Braunschweiger´, ´Copenhaški´, ´Ditmar´, ´Slava´) and five domestic varieties (´Čepinski´, ´Varaždinski´, ´Ogulinski´, ´Žminjski´, ´Brgujski´) were analyzed. Germplasm variability was studied using AFLP molecular markers to be able to determine variability within cultivars and between 9 cultivars. Determining variability is the goal of this research. To determine variability, we used molecular results for the analyzed cultivars, and compared and estimated variability at the molecular level. Seeds were sown in early October 2019, and the seeds were sown in containers and stored under controlled conditions. Of the 9 cultivars studied, 20 individuals were analyzed within each cultivar, and a total of 180 individuals (9 cultivars x 20 individuals). AFLP analysis was done using four AFLP primer combinations. Primary DNA isolation was required, and later this helped in the analysis of the genetic structure for which AFLP markers were used. The diversity matrix was used in molecular variance analysis (AMOVA), cultivar distance analysis, and STRUCTURE analysis.

• Keywords: Brassica oleracea var. capitata; genetska varijabilnost; AFLP; AMOVA; STRUCTURE
• Keywords (english): Brassica oleracea var. capitata; genetic variability; AFLP; AMOVA; STRUCTURE
• Language: croatian
• URN:NBN: urn:nbn:hr:204:611556
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-22 08:51:14