Utjecaj predsjetvenih tretmana na klijavost sjemena i morfologiju klijanaca crnog kima u uvjetima osmotskog stresa

master's thesis

• Title (english): Effect of priming treatmens on seed germination and seedling morphology of Black cumin under osmotic stress conditions
• Author: Ana Trubelja
• Mentor: Martina Grdiša (mentor)
• Committee member: Martina Grdiša (predsjednik povjerenstva)
• Committee member: Boris Lazarević (član povjerenstva)
• Committee member: Klaudija Carović-Stanko (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant biodiversity); Zagreb
• Defense date and country: 2018-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Crni kim (Nigella sativa L.) jednogodišnja je biljna vrsta iz porodice žabnjakovki (Ranunculaceae Juss.), a u narodu je još poznata i pod nazivima crni kumin i ćukerot. Idealnim klimatskim uvjetima smatraju se hladna i suha područja s malim količinama padalina, ali sama oskudica vode nikako nije prikladna za uzgoj ove vrste. Kako se poljoprivreda i poljoprivredna proizvodnja u zadnjih godina sve više susreće s nedostatkom vlage, javlja se potrebe za primjenom određenih tretmana (predsjetvenih tretmana) kojima bi se sjeme moglo pripremiti za takve uvijete. S ciljem utvrđivanja utjecaja predsjetvenih tretmana na klijavost sjemena u uvjetima osmotskog stresa postavljen je dvofaktorijelni pokus na Agronomskom fakultetu Sveučilište u Zagrebu. Ispitana je učinkovitost predsjetvenih tretmana sjemena (močenje sjemena u GA3, koncentracije 100mg/l te dH2O kroz 24h) na parametre klijavosti sjemena i morfologiju klijanaca u uvjetima osmotskog stresa (-3 i -5 bara), simuliranog primjenom PEG 6000. U uvjetima osmotskog stresa nije zamijećeno klijanje sjemena. U uvjetima bez osmotskog stresa, značajno najveća ukupna klijavost od 60% utvrđena je kod tretmana s GA3 (T1). Tretman s destiliranom vodom (T4) i kontrola (T7) imali su manju ukupnu klijavost, odnosno 13,75 i 11,25%, a između njih nije utvrđena značajna razlika. Značajne razlike u morfološkim svojstvima korijena klijanaca utvrđene su kod svojstva duljine i površine, pri čemu su vrijednosti oba svojstva bile značajno veće kod tretmana s GA3 (T1) u odnosu na tretman s destiliranom vodom (T4), ali se nisu značajno razlikovale od kontrole (T7).

Abstract (english)

Black cumin (Nigella sativa L.) is an annual plant species from the family Ranunculaceae Juss, also known by the common names black cumin and ćukerot. Ideal climate conditions for its production are cold and dry areas with lesser amounts of rainfall; however, water stress conditions are not suitable for its cultivation. In recent years agricultural production is faced with drought and it is necessary to apply certain treatments that would enhance seed germination in such conditions. A two-factorial experiment was conducted at the Faculty of Agriculture, University of Zagreb in order to determine the influence of priming treatments on seed germination and seedling morphology in osmotic stress (-3 and -5 bar) conditions, simulated with the application of PEG 6000. The priming treatments tested were: soaking in GA3 (100 mg/l) for 24 h and soaking in dH2O for 24 h. In the osmotic stress conditions seed did not germinate. In conditions without osmotic stress the significantly highest germination percentage of 60% was determined for GA3 (T1) treatment. Treatment with distilled water (T4) and control (T7) had lower total germination, i. e. 13.75 and 11.25%, and no significant differences were found between them. Significant differences in the morphological properties of the root of the seedlings were found for the length and the surface area. The values of both features were higher in treatment with GA3 (T1), when compared to the treatment with distilled (T4), however, it did not significantly differ from the control (T7).

• Keywords: crni kim; Nigella sativa L.; osmotski stres; timokinon; predsjetveni tretmani
• Keywords (english): black cumin; Nigella sativa L.; osmotic stress; timoquinon; seed priming
• Language: croatian
• URN:NBN: urn:nbn:hr:204:536330
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-10-04 07:56:27