Odlike bakterioloških izolata iz lista jagode

master's thesis

• Title (english): Bacterial characteristics of isolates from strawberry leaves
• Author: Dorotea Brajković
• Mentor: Edyta Đermić (mentor)
• Committee member: Edyta Đermić (predsjednik povjerenstva)
• Committee member: Darko Vončina (član povjerenstva)
• Committee member: Martina Skendrović Babojelić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2018-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Ozbiljne probleme u proizvodnji jagode čine različiti uzročnici bolesti. Najčešće i najpoznatije bolesti na jagodama uzrokuju mnogobrojne vrste fitopatogenih gljiva. Virusne i bakterijske bolesti su također prisutne i mogu dovesti do ozbiljnih ekonomskih šteta čije posljedice se lako mogu uvećati neznanjem o uzgoju jagoda. Pojedine fitopatogene bakterije nije lako otkriti. Katkada su njihovi simptomi nevidljivi. U ovom radu izvršena je izolacija svih bakterija iz simptomatičnog tkiva zaraženih listova jagode. Dobiveno je 10 različitih bakterijskih kolonija. Svakom izolatu određena je Gram reakcija i učinjen je test na fluorescenciju. Provedena je hipersenzitivna reakcija na duhanu (Nicotiana tabacum var. 'Samsun') i utvrđena je patogenost izolata 6 te je potom učinjen bio-test na sadnicama jagode. Nakon sedam dana od provedene inokulacije, nije potvrđeno postojanje simptoma nastalih od bakterijskih suspenzija istraživanih izolata.

Abstract (english)

Serious problems in strawberry cultivation can be caused by various plant pathogens. The most common and the most destructive diseases on the strawberries come from many types of phytopathogenic fungi. Viral and bacterial diseases are also present and can cause serious economic damage whose consequences can easily be increased by ignoring strawberry farming.
Certain phytopathogenic bacteria are not easily detected. Sometimes their symptoms are invisible. This research carried out the isolation of all bacteria from the symptomatic strawberry tissue and found 10 different bacterial colonies. Each assay determined the Gram reaction and a fluorescence test was made. A hypersensitive reaction to tobacco (Nicotiana tabacum var. 'Samsun') was performed and the pathogenicity of isolate 6 was induced. A bio-test was carried out on strawberry seedlings. After seven days of inoculation, no symptoms of bacterial suspensions of the tested isolates were confirmed.

• Keywords: Fragaria x ananassa Duch.; patogeni; hipersenzitivna reakcija; detekcija
• Keywords (english): Fragaria x ananassa Duch.; pathogens; hipersentitive reaction; detection
• Language: croatian
• URN:NBN: urn:nbn:hr:204:256352
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-23 11:53:52