Utjecaj porijekla i starosti sjemena na vijabilnost sjemena korovne vrste Ambrosia artemisiifolia

master's thesis

• Title (english): Influence of the seed age and origin on the Ambrosia artemisiifolia L. seed viability
• Author: Mirela Jaganjac
• Mentor: Maja Šćepanović (mentor)
• Committee member: Maja Šćepanović (predsjednik povjerenstva)
• Committee member: Milan Poljak (član povjerenstva)
• Committee member: Klara Barić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Herbology); Zagreb
• Defense date and country: 2017-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Ambrosia artemisiifolia L. jednogodišnja je korovna vrsta iz porodice Asteraceae. Zbog kompeticijskih sposobnosti nanosi znatne direktne štete poljoprivredi ali i indirektne štete proizvodnjom alergenog peluda. Stoga se za ambroziju navodi da je najvažnija korovna vrsta današnjice. Kao monokarpna vrsta razmnožava se samo sjemenom koje zbog svojstva dormantnosti ostaje vijabilno u tlu i nekoliko desetaka godina. Većina jedinki ove vrste nalazi se ispod površine tla, u banci sjemena. Sjeme u banci sjemena može biti dormantno, mrtvo ili živo. Da bi utvrdili u kojem je stanju sjeme proveli smo dva pokusa: test klijavosti na filter papiru sa sjemenom ambrozije koje je uzeto s biljaka i drugi test klijavosti na kvarcnom pijesku sa sjemenom uzetim iz banke sjemena. Osim testa klijavosti, korišten je i TTC test. TTC podrazumijeva korištenje trifenil tetrazolium klorida koji omogućava detekciju dijelova tkiva u kojima se odvija stanično disanje. Cilj je bio utvrditi vijabilnost populacije sjemena korovne vrste Ambrosia artemisiifolia. Nakon provedenog istraživanja u laboratorijskim uvjetima utvrđeno je da starošću sjemena vijabilnost sjemena ambrozije opada.

Abstract (english)

Ambrosia artemisiifolia L. is annual invasive plant from Asteraceae family. Because of its competitive abilities it can cause significant direct damage in yield loss and indirect damage by producing allergenic pollen. As a result, common ragweed is listed as the most important agricultural weed of today. As monocarpic species it reproduces only by seeds which are dormant and these seeds can persist for tens of years in the soil seed bank. Seed can be dormant, dead or alive. To determine condition of the seeds we tested them through two experiments: germination test on a filter paper with seed taken from plants and second germination test on quartz sand with seed from the soil seed bank. It is also used TTC, or triphenyltetrazolium chloride test which is used to detect cellular respiration in parts of the tissue. Our aim was to determine viability of the seeds. After laboratory research it is established that viability of common ragweed decreases with age.

• Keywords: ambrozija; banka sjemena; dormantnost; starost sjemena; vijabilnost sjemena
• Keywords (english): common ragweed; seed bank; dormancy; seed age; seed viability
• Language: croatian
• URN:NBN: urn:nbn:hr:204:581442
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-07-27 06:52:17