Promjene sadržaja produkata fermentacije vodotopivih ugljikohidrata tijekom siliranja različitih hibrida kukuruza

master's thesis

• Title (english): Changes in the contents of carbohydrate fermentation products during ensiling different maize hybrids
• Author: Jelena Marot
• Mentor: Kristina Kljak (mentor)
• Committee member: Kristina Kljak (predsjednik povjerenstva)
• Committee member: Darko Grbeša (član povjerenstva)
• Committee member: Marina Vranić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Animal Nutrition); Zagreb
• Defense date and country: 2017-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Na hranidbenu vrijednost kukuruzne silaže može utjecati cijeli niz čimbenika od izbora hibrida do upotrebe aditiva. Cilj ovog istraživanja je bio istražiti utjecaj hibrida, dodatka inokulanta i vremena siliranja na sadržaj izabranih vodotopivih spojeva. Za pripremu silaža tri hibrida kukuruza (Bc 418b, Bc 678 i Bc exp6) su zasijana u split plot shemi u pet ponavljanja na istom testnom polju, a za analizu su uzeti uzorci zelene mase za siliranje, te silaže nakon 21. i 60. dana siliranja. Sadržaj istraživanih vodotopivih spojeva određen je u vodenom ekstraktu, pri čemu je korištena antron metoda za određivanje sadržaja vodotopivih ugljikohidrata, a enzimatski kitovi za određivanje sadržaja octene kiseline i etanola. Mliječna kiselina je određena spektrofotometrijski i izražena kroz odnos mliječne i octene kiseline. Hibrid je jedino utjecao (P<0,05) na sadržaj vodotopivih ugljikohidrata dok je kod ostalih parametara zabilježen utjecaj inokulanta i vremena siliranja. Tijekom siliranja opadao je sadržaj vodotopivih ugljikohidrata a rastao sadržaj octene kiseline i etanola te odnosa mliječne i octene kiseline. Sadržaj octene kiseline i etanola značajno ispod gornjih optimalnih granica upuduju na optimalne uvjete tijekom siliranja.

Abstract (english)

There are multiple factors from hybrid selection to inoculant addition influencing nutritional value of maize silage. The aim of this research was to explore effects of hybrid, inoculant addition and ensiling time on contents of selected water-soluble compounds. Maize hybrids (Bc 418b, Bc 678 and Bc exp6) selected for ensiling were plantedin a split plot scheme in five repetitions on the same test field; fresh material for ensiling as well as silage after 21st and 60th day of theensiling processwere sampled for analysis. The content of analysed water-soluble compounds was determined in the water extract; antron method was used to determine the content of water-soluble carbohydrate while enzyme kits were used to determine contents of acetic acid and ethanol. Lactic acid was measured spectrophotometrically and expressed through the ratio of lactic and acetic acid. Hybrid affected (P<0.05)only content of water-soluble carbohydrates while other parameters were affected by inoculant addition and the ensiling time. During ensiling process content of water-soluble carbohydrates decreased, while acetic acid, ethanol and lactic acid/acetic acid were increasing. The content of acetic acid and ethanol significantly under the upper optimal limit suggests optimal conditions during the ensiling process.

• Keywords: kukuruzna silaža; hibrid; inokulant; vodotopivi ugljikohidrati
• Keywords (english): corn silage; hybrid; inoculant; water-soluble carbohydrates
• Language: croatian
• URN:NBN: urn:nbn:hr:204:004181
• Study programme: Title: Animal Nutrition and Feed Science; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane (magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-07-07 12:14:26