Title Entomopatogeni potencijal mikrogljive Beauveria bassiana u suzbijanju dlakavog ružičara (Tropinota hirta) Title (english) Entomopathogenic potential of the microfungus Beauveria bassiana in the control of the Apple blossom beetle (Tropinota hirta) Author Matea Vojnović Mentor Katarina Martinko (mentor)Committee member Katarina Martinko (predsjednik povjerenstva)Committee member Ivan Juran (član povjerenstva)Committee member Joško Kaliterna (član povjerenstva)Granter University of Zagreb Defense date and country 2025-01-28, Croatia Scientific / art field, BIOTECHNICAL SCIENCES Abstract Problemi u fitomedicini uzrokovani primjenom kemijskih sredstava za zaštitu bilja doveli su do primjene entomopatogenih organizama u biološkom suzbijanju štetnih organizama. Najčešće korištena entomopatogena gljiva Beauveria bassiana ima entomopatogeno i antagonističko djelovanje, a može biti i saprofit i endofit, stoga ne čudi što se široko primjenjuje. Dlakavi ružičar kornjaš je koji na voćkama, ukrasnim vrstama i ratarskim kulturama uzrokuje značajne štete oštećivanjem cvjetova što dovodi do
... More nemogućnosti zametanja plodova biljaka. Zbog potrebe za suzbijanjem u vrijeme cvatnje, i prisutnosti oprašivača, primjena kemijskih insekticida nije ekološki prihvatljiv izbor pa se odabire biološko suzbijanje entomopatogenom gljivom B. bassiana.
Istraživanje učinkovitosti autohtonog izolata B. bassiana (LD 01) provedeno je u polukontroliranim poljskim uvjetima nasada crnoplodne aronije (Aronia melanocarpa), provođenjem dvaju pokusa s različitim tretmanima odraslih oblika vrste Tropinota hirta (inokulacijski tretman ''dip'' metodom i metodom ''kontaminiranog cvata''). Laboratorijskom metodom inkubiranja uginulih jedinki u vlažnoj komori, stimuliran je rast i razvoj micelija gljive, a infekcija vrstom B. bassiana je potvrđena stereo i svjetlosnim mikroskopom na temelju morfoloških ključeva za determinaciju.
Nakon 14 dana, u pokusu u kojem je korištena ''dip'' metoda, zabilježen je moralitet T. hirta od 23 %, dok je mortalitet u pokusu u kojem je korištena metoda ''kontaminiranog cvata'' iznosio 13 %. Pretpostavlja se da je odgoda i/ili izostanak infekcije sporama B. bassiana posljedica inhibitornog učinka određenih uvjeta okoline (temperatura i vlaga zraka, UV itd.), izolata gljive, morfoloških obilježja i ponašanja dlakavog ružičara tijekom migracije (ukopavanje u tlo/ishrana na cvatu). Obzirom da pregled literature ne omogućuje mnogo dostupnih rezultata o entomopatogenom učinku B. bassiana na T. hirta, a usporedba rezultata s rezultatima laboratorijskih istraživanja na tu temu nije mjerodavna, potrebna su detaljnija istraživanja kako bi se potvrdile navedene pretpostavke. Zbog uočenog velikog broja varijabli koje su potencijalno mogle utjecati na smanjenje infekcije dlakavog ružičara u polukontroliranim poljskim uvjetima nasada, u budućnosti je potrebno provesti istraživanja uz korištenje referentnog izolata B. bassiana kako bi se učinak autohtonog izolata mogao u potpunosti vrednovati. Less Abstract (english) Problems in phytomedicine caused using chemical plant protection products have led to the use of entomopathogenic organisms in the biological control of harmful organisms. The most used entomopathogenic fungus Beauveria bassiana has entomopathogenic and antagonistic effects, and can be both saprophytic and endophytic, so it is not surprising that it is widely used. The apple blossom beetle is a beetle that causes significant damage to fruit trees, ornamental species and field crops by damaging
... More flowers, which leads to the inability to set fruit. Due to the need for control during flowering and the presence of pollinators, the use of chemical insecticides is not an environmentally acceptable choice, so biological control with the entomopathogenic fungus B. bassiana is chosen.
The investigation of the efficacy of the indigenous isolate B. bassiana (LD 01) was conducted in semi-controlled conditions of black chokeberry (Aronia melanocarpa) plantation, by conducting two experiments with different treatments of adult forms of the species Tropinota hirta (inoculation treatment using the ''dip'' method and the ''contaminated inflorescence'' method). The growth and development of the fungal mycelium was stimulated by the laboratory method of incubating dead insects in a humid chamber, and infection with the species B. bassiana was confirmed by stereo and light microscopy based on morphological keys for determination.
After 14 days, in the experiment using the ''dip'' method, the morality of T. hirta was recorded at 23 %, while the mortality in the experiment using the ''contaminated inflorescence'' method was 13 %. It is assumed that the delay and/or absence of infection by B. bassiana spores is a consequence of the inhibitory effect of certain environmental conditions (temperature and humidity, UV, etc.), fungal isolates, morphological characteristics and behavior of the apple blossom beetle during migration (burying in the soil/feeding on inflorescences). Given that the literature review does not provide many available results on the entomopathogenic effect of B. bassiana on T. hirta, and comparison of the results with the results of laboratory studies on this topic is not authoritative and the more detailed studies are needed to confirm the above assumptions. Due to the large number of variables observed that could potentially have influenced the reduction of apple blossom beetle infection in semi-controlled field conditions, it is necessary to conduct studies in the future using a reference isolate of B. bassiana to fully evaluate the effect of the autochthonous isolate. Less Keywords
entomopatogena gljiva
Beauveria bassiana
biološko suzbijanje
polukontrolirani poljski uvjeti
dlakavi ružičar
Tropinota hirta
Keywords (english)
entomopathogenic fungus
Beauveria bassiana
biological control
semi-controlled field
conditions
apple blossom beetle
Tropinota hirta
Language croatian URN:NBN urn:nbn:hr:204:028799 Study programme Title: Phytomedicine Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2025-02-11 10:23:50
