Utvrđivanje patogenosti izolata bakterija iz mumificiranog ploda kruške

master's thesis

• Title (english): Determination pathogenicity of bacteria from mummified pear fruits
• Author: Lucija Kljak
• Mentor: Edyta Đermić (mentor)
• Committee member: Edyta Đermić (predsjednik povjerenstva)
• Committee member: Martina Skendrović Babojelić (član povjerenstva)
• Committee member: Snježana Topolovec-Pintarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2016-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Među bakterijama koje zaražavaju jabuku i krušku najdestruktivnija je Erwinia
amylovora. Cilj ovog rada bio je provjeriti patogenost izoliranih bakterijskih izolata iz
mumificiraog ploda kruške s lokacije Orlovac na sjemenjaku jabuke ʹIdaredʹ. Provjera
patogenosti, odnosno bio-test na jabuci proveden je umjetnom infekcijom sjemenjaka jabuke.
Ranije izolirana mješovita bakterijska kultura pročišćena je do bakterijskih linija. Dobivene
čiste linije nisu se razlikovale međusobno, niti prema morfološkim karakteristikama, niti
prema boji. Infekcija sjemenjaka jabuke provedena je inokulacijom svježe bakterijske
suspenzije preko ranjene kore izbojka i preko lista. Već nakon nekoliko dana najbolji
pokazatelj uspješne zaraze je bila pojava eksudata na mjestu inokulacije, a nakon dva tjedna
uočeni su i simptomi paleži izbojka. Eksudat i palež potvrdili su patogenost testiranog izolata
bakterija za koje se opravdano može pretpostaviti da se radi o vrsti Erwinia amylovora.

Abstract (english)

Among others attacking apples and pears, Erwinia amylovora is the most destructive
bacteria. The aim of this research was to test pathogenicity of isolated bacterial isolates
obtained from mummified pear from location Orlovac on seedling apple. Pathogenicity
testing, i.e. bioassay on apple, was performed as an artificial infection on seedling apple
ʹIdaredʹ. Previously isolated bacterial culture was purified to obtain pure bacterial isolate.
Isolated pure lines were not differentiated in morphological characteristics or color. Seedling
apple infection was performed with inoculation of freshly prepared bacterial suspension over
the branches peel shoot and the leaves. The best indicator of successful infection was exudate
on the spots of inoculation which appeared only several days after, while symptoms of Fire
Blight on branches appeared one week after inoculation. Exudate and Fire Blight symptoms
confirmed pathogenicity of tested bacterial isolate for which it could be justifiable assumed
that is species Erwinia amylovora.

• Keywords: patogenost; bakterijska palež; Erwinia amylovora; bio test; jabuka
• Keywords (english): pathogenicity; Fire Blight; Erwinia amylovora; bioassay; apple
• Language: croatian
• URN:NBN: urn:nbn:hr:204:435536
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-12-13 08:06:21