Mikorizni potencijal mikrogljive Beauveria bassiana na rast i razvoj salate

master's thesis

• Title (english): Mycorrhizal potential of the microfungus Beauveria bassiana on the growth and development of lettuce
• Author: Martin Šutalo
• Mentor: Katarina Martinko (mentor)
• Committee member: Katarina Martinko (predsjednik povjerenstva)
• Committee member: Joško Kaliterna (član povjerenstva)
• Committee member: Ivan Juran (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2024-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Mikrogljiva Beauveria bassiana poznata je kao insekticidno sredstvo, ali novija istraživanja impliciraju da stvara interakciju s biljnim domaćinom sličnu mikorizi. Dosadašnja istraživanja potvrđuju navedenu interakciju u nasadu jagode, grahu, rajčici, kupusu, krastavcima, u usjevima ječma, pšenice i kukuruza, vinove loze, ali istraživanja u uzgoju salate su oskudna. Sukladno navedenom, cilj istraživanja je testirati ''interakciju sličnu mikorizi'' autohtonog izolata B. bassiana na rast i razvoj salate (Lactucae sativa, sorta Ostralie RZ) u uvjetima plastenika.
Višekratna aplikacija suspenzije konidija B. bassiana provedena je u fenofazi razvijena 2-3 lista salate (BBCH 12-13), zalijevanjem biljaka u zonu korijena kroz pet tjedana. Kako bi se kvantificirao potencijal B. bassiana na promociju rasta salate putem potencijalne mikorizne interakcije, izvagana je biomasa te izmjerena površina rozete i korijena svake biljke.
Rezultati potvrđuju značajno povećanje površine korijena biljaka (za 31,6 % i 30,5 %) tretiranih suspenzijom konidija u odnosu na kontrolnu skupinu, kada su biljke tretirane svaki i svaki drugi tjedan. Značajno povećanje biomase biljaka (za 16,7 % i 20 %) zabilježena je u svim test varijantama u odnosu na kontrolu. Površina rozete tretiranih biljaka nije se značajno razlikovala u odnosu na one iz kontrolne skupine. Re-izolacijom mikrogljive iz korijena biljaka tretiranih svaki i svaki drugi tjedan, potvrđen je potencijal B. bassiana da promovira rast salate (kao posljedica interakcije slične mikorizi) u uvjetima plastenika, ali i da nakon 10 tjedana više nije prisutna u koloniziranim biljkama.
Dobiveni rezultati su potvrda suvremenih istraživanja koja pokazuju slične rezultate na raznim biljnim kulturama. Također, B. bassiana pokazuje sposobnost interakcije slične mikorizi s korijenom salate, no potrebna su dodatna istraživanja u uvjetima in vivo u svrhu boljeg razumijevanja ovog oblika interakcije.

Abstract (english)

The microfungus Beauveria bassiana is known as an insecticidal agent, but recent studies imply that it forms a mycorrhiza-like interaction with the plant host. Previous studies confirm the mentioned interaction in strawberry, bean, tomato, cabbage, cucumber, barley, wheat and corn crops, vines, but studies in lettuce cultivation is scarce. The goal of the research is to test the "mycorrhiza-like interaction" of the autochthonous isolate B. bassiana on the growth and development of lettuce (Lactucae sativa, variety Australiae RZ) in greenhouse conditions.
Repeated application of B. bassiana conidia suspension was carried out in the phenophase of 2-3 lettuce leaves (BBCH 12-13), by watering the plants in the root zone for five weeks. In order to quantify the potential of B. bassiana to promote lettuce growth through a potential mycorrhizal interaction, the weight and rosette and roots area of each plant were weighed.
The results confirm a significant increase in the root area of the plants (by 31.6% and 30.5%) treated with the conidia suspension, compared to the control group, when the plants were treated every and every other week. Significantly higher biomass of plants (by 16.7% and 20%) was recorded in all test variants compared to the control. The rosette area of the treated plants did not differ significantly compared to those from the control group. By re-isolating the microfungus from the roots of plants treated every and every other week, the potential of B. bassiana to promote the growth of lettuce (as a result of mycorrhizal-like interaction) in greenhouse conditions was confirmed but also that it did not persist in the plants after 10 weeks. The obtained results are a confirmation of modern studies that shows similar results on various plant species. Also, B. bassiana shows the ability to interact like mycorrhizae fungi with lettuce roots, but additional research in in vivo conditions is needed to better understand this form of interaction.

• Keywords: Beauveria bassiana; mikoriza; endofit; in vivo; suspenzija spora; salata
• Keywords (english): Beauveria bassiana; mycorrhiza; endophyte; in vivo; spore suspension; lettuce
• Language: croatian
• URN:NBN: urn:nbn:hr:204:600594
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-18 07:02:22