Genomska procjena inbridinga na temelju markera spolnih kromosoma

master's thesis

• Author: Martina Šantek
• Mentor: Ino Čurik (mentor)
• Committee member: Ino Čurik (predsjednik povjerenstva)
• Committee member: Miroslav Kapš (član povjerenstva)
• Committee member: Vlatka Čubrić Čurik (član povjerenstva)
• Committee member: Maja Ferenčaković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Animal Science); Zagreb
• Defense date and country: 2016-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy; Genetics and Breeding of Plants, Animals and Microorganisms

Abstract

Inbriding, odnosno parenje jedinki u srodstvu se mjeri koeficijentom inbridinga, F. Do
vrijednosti koeficijenta inbridinga se može doći na više načina, jedan od kojih je procjena
inbridinga pomoću određivanja homozigotnih odsječaka (ROH). Genomski koeficijent
inbridinga se obično odnosi na autosome, dok se inbriding na X kromosomu zanemaruje. U
ovom radu su određeni ROH-ovi >4 Mb, >8 Mb i >16 Mb kod jedinki populacije Soay ovaca
genotipiziranih Ovine 50K SNP čipom te su određene vrijednosti koeficijenata inbridinga za
autosome i X kromosom. Za autosome su određene vrijednosti ukupno za sve kromosome te
posebno za svaki kromosom. Inbriding na X kromosomu je ~4 do 15 puta veći nego na
autosomima, a vrijednosti za autosomalni i X inbriding uvelike razlikuju kod pojedinih jedinki
te je korelacija između vrijednosti niska. X inbriding može biti važan kod vrsta kojima veći dio
genoma čini X kromosom te kod kojih su neka važna svojstva djelomično određena genima na
X kromosomu te stoga ne smije biti zanemarivan.

Abstract (english)

Inbreeding or mating of related individuals is measured by the coefficient of inbreeding, F.
There are more ways to calculate the coefficient of inbreeding, one of which is estimating
inbreeding with use of homozygous runs (ROHs). Genomic inbreeding coefficient is usually
based on the autosomal chromosomes, while the inbreeding on the X chromosome is neglected.
In this thesis, ROHs>4 Mb, >8 Mb and >16 Mb are detected in individuals from Soay sheep
population genotyped with Ovine 50K SNP chip and coefficients of inbreeding are determined
for autosomal chromosomes and X chromosome. In addition, coefficients of inbreeding are
determined for every autosomal chromosome, separately. Detected inbreeding on X
chromosome is ~4 to 15 times higher than on the autosomal chromosomes, and values for
autosomal and X-chromosomal inbreeding differ a lot in individuals and the correlation
between two values is low. X inbreeding can be important in species that have most of their
genome located on the X chromosome and those who have some important traits partially
determined by genes on X chromosome and therefore must not be neglected.

• Keywords: inbriding; autosomalni inbriding; X inbriding; ROH
• Keywords (english): inbreeding; autosomal inbreeding; X inbreeding; ROH
• Language: croatian
• URN:NBN: urn:nbn:hr:204:231168
• Study programme: Title: Animal Genetics and Breeding; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja (magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-11-30 07:21:57