Utjecaj gljiva potencijalnih promotora biljnog rasta na korijenov sustav kupusa

master's thesis

• Title (english): The impact of potential plant growth-promoting fungi on the root system of cabbage
• Author: Lucija Batistić
• Mentor: Ivana Rajnović (mentor)
• Mentor: Ines Sviličić Petrić (komentor)
• Committee member: Ivana Rajnović (predsjednik povjerenstva)
• Committee member: Ines Sviličić Petrić (član povjerenstva)
• Committee member: Boris Lazarević (član povjerenstva)
• Committee member: Marko Petek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2023-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

U rizosferi tla, među brojnim mikroorganizmima, zastupljene su tzv. PGP (eng. Plant growth promoting) gljive koje mogu raznim mehanizmima poticati rast biljaka. U ovom radu identificirano je 40 izolata gljiva koji su u prethodnim mikrobiološkim ispitivanjima pokazali da posjeduju željena PGP svojstva. Sekvenciranjem ITS genske regije, utvrđeno je da većina izolata pripada rodovima Penicillium (25 % izolata) te Aspergillus (20 % izolata). U laboratorijskim uvjetima ispitan je njihov utjecaj na rast korijena kupusa. Statističkom obradom podataka utvrđeno je da su najučinkovitiji bili izolati P2.10 – 3 (Aspergillus clavatus) P1. 15 – 1 (Penicillium brevicompactum) te P1.15 – 2 (Penicillium sp.). Ovi izolati nosioci su tri (P1.15 – 1, P1.15 – 2) do pet (P2.10 – 3) PGP svojstava te su izolirani iz tala koja su prethodno bila izložena poplavama. Zbog toga bi se daljnja istraživanja trebala usmjeriti na njihovu potencijalnu primjenu u zaštiti bilja od ekstremnih ekoloških uvjeta.

Abstract (english)

Among numerous groups of microorganisms, rhizosphere is inhabited by Plant Growth Promoting Fungi (PGPF) which stimulate plant growth by various mechanisms. In this thesis, 40 fungal isolates were chosen based on the previous results of microbiological tests, showing that they possess the desired PGP properties. Sequencing the ITS region, revealed that most of the isolates belong to the genera Penicillium (25 % of isolates) and Aspergillus (20 % of isolates). Their influence on the growth of cabbage roots was tested in laboratory conditions. Statistical analysis of the data revealed that the isolates P2.10 – 3 (Aspergillus clavatus) P1.15 – 1 (Penicillium brevicompactum) and P1.15 – 2 (Penicillium sp.) were the most effective. These isolates were found to possess three (P1.15 – 1, P1.15 – 2) to five (P2.10 – 3) PGP traits and were isolated from soils previously exposed to floods. For this reason, further research should be focused on their potential application in protecting plants from extreme environmental conditions.

• Keywords: PGP gljive; rizosfera; klimatske promjene; Brassica oleracea var. capitata
• Keywords (english): PGP fungi; rhizosphere; climate change; Brassica oleracea var. capitata
• Language: croatian
• URN:NBN: urn:nbn:hr:204:670943
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2023-10-31 08:07:53