Fenotipska i genotipska karakterizacija gljiva povezanih sa sušenjem šipka (Punica granatum L.) u Dalmaciji

master's thesis

• Title (english): Phenotype and genotype characterization of fungi associated with pomegranate (Punica granatum L.) dieback in Dalmatia
• Author: Lucija Šušić
• Mentor: Joško Kaliterna (mentor)
• Committee member: Joško Kaliterna (predsjednik povjerenstva)
• Committee member: Snježana Topolovec-Pintarić (član povjerenstva)
• Committee member: Jelena Gadže (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Pathology); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Na nekoliko lokaliteta na području Dalmacije tijekom 2019. godine su uočene bolesne biljke šipka čiji su simptomi upudivali na citosporiozni rak i venude. U narednim je istraživanjima potvrđeno da se radi o vrsti Cytospora punicae za koju je pretpostavljeno da je glavni uzročnik propadanja šipka. Bududi da je šipak cijenjena mediteranska vodna vrsta s visokim potencijalom uzgoja u Dalmaciji, potrebno je dodatno istražiti njezin fitopatološki status u Hrvatskoj. Fitopatogene gljive su najvažniji i najčešdi uzročnici bolesti šipka, s posebnim naglaskom na uzročnike bolesti drva. Njihova prisutnost, osobito na oslabjeloj biljci, može dovesti do propadanja cijele biljke. U prethodnim istraživanjima je iz drva šipka sa simptomima venuda izolirano nekoliko gljiva među kojima je jedina identificirana vrsta bila spomenuta Cytospora punicae. U ovom su istraživanju analizom DNA sekvenci molekularnih markera ITS i EF1-α primjenom PCR metode identificirane još tri nove vrste za koje se smatra da uz vrstu C. punicae uzrokuju propadanje šipka. Dobivene ITS i EF1-α sekvence su uspoređene s odgovarajudim sekvencama iz GenBank baze podataka primjenom bioinformatičkog programa BLAST, a za filogenetsku analizu je korištena metoda Maximum Likelihood. Reprezentativni su izolati okarakterizirani fenotipski, analizom morfoloških karakteristika i testom patogenosti na šipku.

Abstract (english)

During 2019, on few sites across Dalmatia were observed pomegranate plants that were indicating Cytospora canker and wilting symptoms. Further research determined a Cytospora punicae species from collected wood, persumed to be the main cause of pomegranate decay. Pomegranate is high valued mediterranean species with excelent cultivation potential across region of Dalmatia, therefore further investigation of its phytopathological status in Croatia is crucial. The most important and most common causes of pomegranate diseases are phytopathogenic fungi, with emphasis on the causative agents of wood diseases. Their presence leads to the decay of the whole plant, especially targeting the weakened plants. In previous studies several fungi were isolated from pomegranate plants which indicated wilting symptoms. At the time, the only indentified fungi was Cytospora punicae. In this study, by using PCR method to analyze DNA sequences of molecular markers ITS and EF1-α, three new species were identified. The new species are belived to cause pomegranate decay in addition to C. punicae. The obtained ITS and EF1-α sequences were compared with the corresponding sequences from the GenBank database using the BLAST bioinformatics program, and the Maximum Likelihood method was used for phylogenetic analysis. Representative isolates are described by phenotype. Morphological analysis and pathogenicity test were conducted on pomegranate.

• Keywords: šipak; fitopatogene gljive; bolesti drva šipka
• Keywords (english): pomegranate; phytopathogenic fungi; pomegranate wood diseases
• Language: croatian
• URN:NBN: urn:nbn:hr:204:426838
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-11-29 11:54:35