Identifikacija gljiva povezanih sa sušenjem sadnica i čokota vinove loze u Hrvatskoj i Bosni i Hercegovini

master's thesis

• Title (english): Identification of Fungi Associated with Dieback of Seedlings and Vines of Grapevine in Croatia and Bosnia and Herzegovina
• Author: Marija Blažević
• Mentor: Joško Kaliterna (mentor)
• Committee member: Joško Kaliterna (predsjednik povjerenstva)
• Committee member: Snježana Topolovec-Pintarić (član povjerenstva)
• Committee member: Marko Karoglan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2021-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Vinova loza je kultura koja ima dugu povijest uzgoja u Hrvatskoj i Bosni i Hercegovini, važna je kultura koja se uzgaja na velikim površinama. Fitopatogene gljive su najvažniji i najznačajniji patogeni vinove loze. Budući da uzrokuju velike štete, u posljednjih nekoliko desetljeća sve veći značaj se posvećuje bolestima drva vinove loze čiji su uzročnici fitopatogene gljive. Tijekom 2019. godine biljni materijal sa simptomima bolesti drva vinove loze zaprimljen je u laboratorij Zavoda za fitopatologiju Agronomskog fakulteta Sveučilišta u Zagrebu. Iz zaprimljenih uzoraka dobivene su čiste kulture gljiva, izolirane gljive zatim su identificirane prema svojim morfološkim obilježjima kolonija i konidija na hranjivoj podlozi PDA. Reprezentativni izolati su podvrgnuti molekularnoj analizi, gdje im je najprije izolirana ukupna genomska DNA, a zatim su metodom lančane reakcije polimerazom (PCR) umnožene ITS regije genoma. PCR produkti nakon umnažanja su sekvencirani, te je utvrđen slijed nukleotida. Sekvence su uspoređene sa odgovarajućim sekvencama pohranjenima u međunarodnoj banci gena GenBank, koristeći program BLAST, a dodatno je provedena i filogenetska analiza kako bi se potvrdila taksonomska pripadnost izolata. Na temelju podataka dobivenih u ovom istraživanju koristeći se svim navedenim metodama identificirane su slijedeće vrste: Dactylonectria pauciseptata i Cytospora vinacea.

Abstract (english)

Grapevine is culture that has long history of cultivation in Croatia and Bosnia and Herzegovina, it is important culture that is cultivated in large areas. Phytopathogenic fungi are most significant and most important pathogens of grapevine. In last few decades Grapevine trunk disease (GTD) that are caused by phytopathogenic fungi are gaining more importance because they are causing significant damages. During 2019. laboratory of Department of plant pathology, Faculty of Agriculture, University of Zagreb, received plant material showing symptoms of GTD. From the samples that were received, clean cultures of fungi were obtained, and isolated fungi were then identified by morphological characteristics of their colonies and conidia on the growth media PDA. Representative isolates were subjected to molecular analysis, where their whole genomic DNA was first isolated, and then using polymerase chain reaction (PCR) ITS regions were amplified. After amplification PCR products were sequenced, and their nucleotide sequence was determined. Sequences were compared using BLAST program, with corresponding sequences from international gens bank GenBank, and additionally phylogenic analysis was conducted for confirmation of taxonomic status of the isolates. Based on all of the data obtained in this research, and using all the methods mentioned above, following species were identified: Dactylonectria pauciseptata and Cytospora vinacea

• Keywords: vinova loza; bolesti drva; fitopatogene gljive; simptomi; identifikacija
• Keywords (english): Grapevine; Trunk disease; phytopathogenic fungi; symptoms; identification
• Language: croatian
• URN:NBN: urn:nbn:hr:204:523178
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2021-10-08 12:37:20