Identifikacija signala pozitivne selekcije u genomu hrvatskih ovaca

master's thesis

• Title (english): Detection of positive selection signatures in the genome of Croatian sheep
• Author: Mario Shihabi
• Mentor: Ino Čurik (mentor)
• Committee member: Ino Čurik (predsjednik povjerenstva)
• Committee member: Vlatka Čubrić Čurik (član povjerenstva)
• Committee member: Maja Ferenčaković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Animal Science); Zagreb
• Defense date and country: 2021-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Ovčarstvo je jedan od najvažnijih stočarskih sektora u mediteranskom području
Hrvatske odakle potječe većina hrvatskih autohtonih pasmina. Prisutni signali pozitivne
selekcije posljedica su adaptacije na oskudne okolišne čimbenike, ali i odabira (selekcije)
ljudi. U ovom istraživanju je uz autosome bio uključen i spolni kromosom X (kod ženskih
jedinki), zbog utjecaja spolno vezanog nasljeđivanja na ekonomski i evolucijski važna
svojstva. Cilj rada bio je identificirati signale pozitivne selekcije putem dvije metode, iHS i
eROHi, koristeći uzorak veličine 102 jedinke iz 4 autohtone pasmine ovaca Hrvatske i 606
006 SNP-ova (Infinium® HD SNP BeadChip), na kojem je prije analize obavljena kontrola
kvalitete. Obje metode temelje se na analizi genomskih varijacija unutar jedne velike metapopulacije,
koja ovdje predstavlja hrvatske autohtone pasmine ovaca. Uočeni su mnogi
ekonomski i evolucijski važni geni te je tako razjašnjena prisutnost pojedinih signala u
populaciji. Za precizniju i pouzdaniju analizu potrebno je povećati uzorak te uključiti još
metoda identifikacije.

Abstract (english)

Sheep farming is one of the most important livestock sectors in the Mediterranean
region of Croatia, where most of the Croatian autochthonous breeds originate from. The
present positive selection signatures are a consequence of adaptation to scarce
environmental factors, but also of artificial selection. In addition to autosomes, sex
chromosome X (in females) was included in this study because of the known influence of
sex-linked inheritance on many economically and evolutionarily important traits. The aim of
this study was to identify positive selection signatures by two methods, iHS and eROHi,
using a sample size of 102 individuals from 4 Croatian autochthonous breeds and 606 006
SNPs (Infinium® HD SNP BeadChip) on which quality control was performed before analysis.
Both methods are based on the analysis of genomic variation within a large metapopulation,
which here represents Croatian autochthonous sheep breeds. Many
economically and evolutionarily relevant genes were identified, highlighting the presence of
multiple signatures in the population. For a more accurate and reliable analysis, it is
necessary to increase the sample size and include additional identification methods.

• Keywords: Hrvatske autohtone pasmine ovaca; Signali pozitivne selekcije; Kandidat geni; Autosomi; Spolni kromosom X
• Keywords (english): Croatian autochthonous sheep breeds; Positive selection signatures; Candidate genes; Autosomes; Sex chromosome X
• Language: croatian
• URN:NBN: urn:nbn:hr:204:179823
• Study programme: Title: Animal Genetics and Breeding; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja (magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-07 11:27:29