Polimorfizam DGAT1 i TG gena u križanaca belgijskog plavog i holštajn goveda

master's thesis

• Title (english): Polymorphism of DGAT1 and TG genes in crossbreeds of Belgian blue and Holstein cattle
• Author: Melinda Kazić
• Mentor: Jelena Ramljak (mentor)
• Committee member: Jelena Ramljak (predsjednik povjerenstva)
• Committee member: Miljenko Konjačić (član povjerenstva)
• Committee member: Krešimir Salajpal (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Animal Science and Technology); Zagreb
• Defense date and country: 2020-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Selekcija u stočarskoj proizvodnji u novije vrijeme provodi na razini gena ili cijelog genoma koristeći različite genetske markere. Cilj ovog rada bio je utvrditi frekvenciju alela i genotipova, genetske parametre i te komparaciju dobivenih rezultata s rezultatima drugih istraživanjima za gene DGAT1 (diacilglicerol O-acilotransferaza) i TG (tiroglobulin) na uzorku križanaca belgijskog plavog i holštajn goveda. Geni DGAT1 i TG navode se kao kandidat geni odgovorni za odlaganje intramuskularne masti i mramoriranosti u mesu goveda. Oba gena u ovom istraživanju bila su polimorfna s frekvencijama alela kako slijedi: DGAT1 (f(K) = 0,551, f(A) = 0,449) i TG gen ( f(T) = 0,112, f(C) = 0,888). Ukupno je utvrđeno pet genotipova, odnosno za DGAT1 gen nije utvrđen genotip AA. Utvrđene frekvencija genotipova križanaca belgijskog plavog i holštajn goveda na lokusu DGAT1 značajno (p<0,01) odstupaju od frekvencije genotipova populacije koja se nalazi u Hardy-Weinbergovoj ravnoteži, dok to nije bio slučaj za TG lokus. Dobiveni genetski parametri polimorfizma DGAT1 i TG gena ovog istraživanja usporedivi su s rezultatima drugi znanstvenih istraživanja.

Abstract (english)

Recently, the selection in the livestock industry is based on the gene or the entire genome using different genetic markers. The goal of this thesis was to determine the frequency of alleles and genotypes, genetic parameters and results comparison of DGAT1 (diacylglycerol-O-acyltransferase 1) and TG (thyroglobulin) genes on Belgian blue and Holstein cattle crossebreeds. The DGAT1 and TG genes are expected to be responsible for intramuscular fat deposition and marbling of meat in cattle. In this research both genes were polymorphic with alleles frequencies as follows: DGAT1 (f(K) = 0,551, f(A) = 0,449) i TG gene ( f(T) = 0,112, f(C) = 0,888). Five genotype frequencies were determined, except AA genotype of DGAT1 gene. Observed genotype frequencies Belgian blue and Holstein cattle crosses
Furthermore, results showed that genotypes of Belgian Blue and Holstein cattle crossbreeds on DGAT1 locus significantly deviate from genotype frequencies of population, which is in Hardy-Weinberg's equilibrium. On the other hand, TG locus did not show such deviation. In conclusion, genetic parameters of DGAT1 and TG polymorphism from this research are comparable to results from other scientific researches.

• Keywords: polimorfizam; DGAT1; TG; križanci belgijskog plavog i holštajn goveda
• Keywords (english): polymorphism; DGAT1; TG; crossbreeds of Belgian blue and Holstein cattle
• Language: croatian
• URN:NBN: urn:nbn:hr:204:538072
• Study programme: Title: Production and Processing of Meat; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka proizvodnje i prerade mesa (magistar / magistra inženjer/inženjerka proizvodnje i prerade mesa)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-10-07 08:12:01