Patogenost bakterijskih izolata iz nekrotičnog tkiva lista kupusa

master's thesis

• Title (english): Pathogenicity of bacterial isolates from cabbage leaves necrotic tissue
• Author: Ivana Ivančić
• Mentor: Edyta Đermić (mentor)
• Mentor: Damir Đermić (mentor)
• Committee member: Edyta Đermić (predsjednik povjerenstva)
• Committee member: Božidar Benko (član povjerenstva)
• Committee member: Damir Đermić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Kupus je jedna od najznačajnijih povrtnih kultura u svijetu, ali i Hrvatskoj, što potvrđuju statistički podaci o njegovom uzgoju i proizvodnji. Važnost kupusa se ogleda u njegovoj nutritivnoj vrijednosti i činjenici da ga, zbog izražene otpornosti na niske temperature i povoljnih uvjeta uzgoja u našoj zemlji, možemo uzgajati tijekom cijele godine te omogućiti konzumaciju kupusa (u svježem stanju ili prerađenom na razne načine) kroz cijelu godinu. Obzirom da bakterioze u povoljnim uvjetima mogu predstavljati ozbiljnu prijetnju i ograničavajući faktor proizvodnje kupusa, cilj rada je bio izolirati fitopatogene bakterije iz nekrotičnih listova kupusa iz Umaga. Konvencionalnim bakteriološkim tehnikama iz tkiva lista kupusa izolirane su bakterije. Bakterijski izolati su pročišćeni i testirani s obzirom na Gram-reakciju, rast na kompletnim i poluselektivnim hranjivim podlogama, te im je provjerena patogenost na duhanu. Detektirana je prisutnost bakterija iz roda Xanthomonas.

Abstract (english)

Cabbage is one of the most important vegetable crops in the world, as well as in Croatia, as confirmed by statistics on its cultivation and production. The importance of cabbage is reflected in its nutritional value and the fact that, due to its high resistance to low temperatures and favorable growing conditions in our country, we can grow it year-round and allow the consumption of cabbage (fresh or processed in various ways) throughout the year. Since bacterial diseases of cabbage, under favorable conditions, can pose a serious threat and limiting factor for cabbage production, the aim of the study was to isolate phytopathogenic bacteria from necrotic cabbage leaves from Umag. The bacteria from cabbage leaf tissue have been isolated by conventional bacteriological techniques. The bacterial isolates were purified and tested for Gram reaction, growth on complete and semi-selective nutrient media, and their pathogenicity in tobacco was tested. Presence of bacteria members of the genus Xanthomonas were detected.

• Keywords: kupus; bakterioze kupusa; Gram reakcija; hipersenzitivna reakcija; rod Xanthomonas
• Keywords (english): cabbage; bacterioses of cabbage; Gram reaction; hypersensitive reaction; genus Xanthomonas
• Language: croatian
• URN:NBN: urn:nbn:hr:204:887496
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-11-26 07:38:29