Utjecaj štetnih mutacija u uzgoju mliječnih goveda s osvrtom na APAF1 gen

master's thesis

• Title (english): Influence of harmful mutations in dairy cattle with a review to APAF1 gen
• Author: Vedrana Popijač
• Mentor: Vlatka Čubrić Čurik (mentor)
• Committee member: Vlatka Čubrić Čurik (predsjednik povjerenstva)
• Committee member: Ino Čurik (član povjerenstva)
• Committee member: Maja Ferenčaković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Animal Science); Zagreb
• Defense date and country: 2019-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Mutacije su bitne za evoluciju pa čak i one štetne. No, većinski mogu uzrokovati velike probleme i gubitke pri uzgoju životinja. Pojava besmislene mutacije na APAF1 genu dovela je do velikih novčanih gubitaka. Poznati bik, Chef, bio je idealna jedinka za stvaranje potomaka, ali je u populaciju uveo i smrtonosni gen odgovoran za oko pola milijuna spontanih pobačaja diljem svijeta. Zahvaljujući metodama sekvenciranja nove generacije pomoću kojih je pronađen uzrok nastalih gubitaka, razvijeni su dijagnostički testovi za mutaciju APAF1 koje uzgajivači mogu koristiti kako bi izbjegli parenje dva nosioca štetnih mutacija, a da pritom zadrže korisne genetske doprinose.

Abstract (english)

Mutations are essential for evolution, even the harmful ones. But mostly they can cause major problems and losses in animal breeding. The appearance of nonsense mutation on the APAF1 gene has led to large losses, population and moneywise. The famous bull, Chef, was the ideal offspring creator, but he also introduced a deadly gene responsible for about half a million spontaneous miscarriages around the world. Thanks to the new generation sequencing methods the cause of the resulting losses was found and the APAF1 mutation diagnostic tests were developed that breeders can use to avoid the mating of two carriers while retaining useful genetic contributions.

• Keywords: mutacija; APAF1 gen; holstein govedo; NGS sekvenciranje
• Keywords (english): mutation; APAF1 gene; Holstein cattle; NGS sequencing
• Language: croatian
• URN:NBN: urn:nbn:hr:204:220852
• Study programme: Title: Animal Genetics and Breeding; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja (magistar/ magistra inženjer/inženjerka genetike i oplemenjivanja životinja)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-11-18 10:20:33