Određivanje broja vijabilnih stanica izolata K16 bakterije Erwinia amylovora u tekućoj kulturi

master's thesis

• Title (english): Determination of the number of viable cells in bacterium Erwinia amylovora isolate K16 in liquid culture
• Author: Andrea Dragoš
• Mentor: Edyta Đermić (mentor)
• Committee member: Edyta Đermić (predsjednik povjerenstva)
• Committee member: Martina Skendrović Babojelić (član povjerenstva)
• Committee member: Nenad Jalšenjak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Plant Pathology); Zagreb
• Defense date and country: 2018-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Bakterija Erwinia amlovora (Burrill) Winslow et. al. uzročnik je najstarije i najdestruktivnije bakterijske bolesti, bakterioznog paleža. Bakteriozni palež destruktivan je na kruškama, a manje na jabuci, dunji i oskoruši. Kao što govori samo ime bolesti, zaraženi biljni dijelovi izgledaju kao opaljeni vatrom. U ovom radu opisani su neki od karakterističnih simptoma ove bolesti, njen životni ciklus, načini suzbijanja kao i prognozni modeli. Glavni cilj ovog rada bio je praćenje brojnosti vijabilnih stanica izolata K16 fitopatogene bakterije Erwinia amylovora u tekućem hranjivom mediju nakon čega se predlažu optimalni termini za uzorkovanje bakterijske kulture namijenjene izvođenju pokusa sa antibakterijskim agensima. Nakon inokulacije, pri uzgoju u tekućem hranjivom mediju, populacija prolazi kroz 5 faza koje su opisane krivuljom rasta bakterijske kulture. Poznato je kako neke stanice u zadnjoj fazi mogu ostati vijabilne, ali prijeđu u stanje mirovanja i ne mogu formirati kolonije.

Abstract (english)

Erwinia amylovora (Burrill) Winslow et. al. is described as the oldest and most destructive bacterial disease, also know as fire blight. Fire blight is destructive to pear and less to apple, quince and medlar. As the name of the disease says, plants look like they were burned by fire. This paper sums up all characteristical symptoms of this disease, its life cycle, models of control as well as models of forecasting. The main purpose of this study was to determinate the number of viable cells in bacterium Erwinia amylovora isolate K16 in liquid culture and to suggest optimal bacterial culture sampling term for antibacterial assays. After inoculation and growing in a liquid nutrient medium the bacterium passes through 5 phases that are described by the growth curve. It has been known that some of the cells in the last phase may remain viable and cannot form colonies.

• Keywords: biljni patogen; krivulja rasta; kolonija; bakteriozni palež; kruška
• Keywords (english): plant pathogen; growth curve; colonies; fire blight; pear
• Language: croatian
• URN:NBN: urn:nbn:hr:204:459562
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-11-07 11:13:58