Ko-inokulacija sjemena soje s autohtonim sojevima Bradyrhizobium japonicum i Pseudomonas fluorescens

master's thesis

• Title (english): Co-inoculation of soybean seed with indigenous strains of Bradyrhizobium japonicum and Pseudomonas fluorescens
• Author: Bernarda Lenkert
• Mentor: Sanja Sikora (mentor)
• Committee member: Sanja Sikora (predsjednik povjerenstva)
• Committee member: Ana Pospišil (član povjerenstva)
• Committee member: Mihaela Blažinkov (član povjerenstva)
• Committee member: Sanja Kajić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Microbiology); Zagreb
• Defense date and country: 2018-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Soja predstavlja jednu od vodećih ratarskih i ekonomski važnih kultura u svijetu. Predsjetvena bakterizacija soje osigurava opskrbu dušikom prirodnim putem te time umanjuje potrebu za unosom mineralnih gnojiva. Velike klimatske promjene su potaknule selekciju kultivara soje te sojeva kvržičnih bakterija s ciljem prilagodbe novonastalim ekološkim uvjetima. Selekcija autohtonih sojeva kvržičnih bakterija igra važnu ulogu u većem iskorištavanju procesa biološke fiksacije dušika, jer predstavlja vrijedan genentski potencijal određene važne poljoprivredne regije. Pravilan odabir bakterijskog soja osigurava raniju i efikasniju infekciju i nodulaciju te optimalno usvajanje atmosferskog dušika. Cilj ovoga istraživanja je utvrditi utjecaj inokulacije soje sa različitim sojevima kvržičnih bakterija i korisnim bakterijama koje pospješuju rast biljaka, te koji će se od autohtonih sojeva kvržičnih bakterija pokazati najefikasniji u nodulaciji soje. U svrhu istraživanja proveden je pokus u kojemu su korišteni autohtoni sojevi kvržičnih bakterija izolirani sa različitih lokacija Hrvatske te Pseudomonas fluorescens kao bakterija koja promovira rast biljaka. Rezultati istraživanja pokazali su kako je ko-inokulacija kvržičnih i PGPR bakterija primjer iskorištavanja korisnih mikroorganzama u uzgoju soje te se kao takav može smatrati jednim od vrlo značajnih alata biotehnologije u sustavima održive poljoprivrede.

Abstract (english)

Soybean is one of the world's leading agricultural and economically important crops. Soybean inoculation can provide natural supply of plants with nitrogen, thereby reducing the need for input of synthetic fertilizers. Large climate changes have encouraged selection of soybean cultivars and strains of nodule bacteria that would adapt to new ecological conditions. Selection of indigenous rhizobial strains plays an important role in soil microbiology, as it represents valuable genetic potential from a certain agricultural region. Proper bacterial strain selection can provide an earlier and more effective infection, nodulation and fixation of atmospheric nitrogen. The aim of this research was to determine the impact of soybean inoculation with different strains of nodule and PGPR bacteria, and to establish which of the indigenous strains will provide the most effective soybean nodulation. For this purpose vegetation pot experiment was conducted in which indigenous rhizobial strains isolated from different regions Croatia and bacterium with plant growth promoting abilities – Pseudomonas flurescens were tested. The result of the study showed that soybean co-inoculation with nodule bacteria and PGPR is a example for positive relationship between microorganisms and legumes that can be considered as one of the important tools of biotechnology in sustainable agricultural systems.

• Keywords: soja; Bradyrhizobium japonicum; inokulacija; PGPR; nodulacija; simbiozna učinkovitost
• Keywords (english): soya bean; Bradyrhizobium japonicum; inoculantion; PGPR; nodulation; symbiotic efficiency
• Language: croatian
• URN:NBN: urn:nbn:hr:204:638935
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologija (magistar/ magistra inženjer/inženjerka agroekologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-10-09 08:08:15