Koncentracija imunoglobulina u kravljem kolostrumu na OPG-u Hubak

master's thesis

• Title (english): Concentracion of immunoglobulin in cow colostrum on familiy farm Hubak
• Author: Ivana Hubak
• Mentor: Miljenko Konjačić (mentor)
• Committee member: Miljenko Konjačić (predsjednik povjerenstva)
• Committee member: Nikolina Kelava Ugarković (član povjerenstva)
• Committee member: Neven Antunac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Animal Science and Technology); Zagreb
• Defense date and country: 2018-06-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Tele se rađa bez vlastitih zaštitnih tvari (imunoglobulina) koje bi ga štitile od patogenih uzročnika bolesti. Stoga je vrlo važno da tele što ranije popije kvalitetan kolostrum koji je bogat imunoglobulinima. Cilj ovog rada bio je istražiti koncentraciju imunoglobulina u kolostrumu te utvrditi utjecaj dobi, redoslijeda laktacije, sezone i pasmine na koncentraciju imunoglobulina. Koncentracija imunoglobulina mjerena je korištenjem dva uređaja: kolostrum denzimetra i refraktometra. Prosječna koncentracija imunoglobulina pri prvoj mužnji mjerena kolostrum denzimetrom iznosila je 68,19 mg/mL, dok je ona mjerena refraktometrom iznosila 56,31 mg/mL. Izmjerene vrijednosti veće su od graničnih vrijednosti (50 mg/mL) tako da je prosječna koncentracija imunoglobulina u kolostrumu na OPG-u Hubak zadovoljavajuća. Utvrđena je visoka pozitivna korelacija između rezultata mjerenja ova dva uređaja (0,80 P<0,0001) tako da se oba uređaja sa jednakom pouzdanošću mogu koristiti za određivanje koncentracije imunoglobulina u kravljem kolostruma. Istraživanjem nije utvrđen značajan utjecaj dobi, redoslijeda laktacije, sezone i pasmine na koncentraciju imunoglobulina kolostruma. Međutim, utvrđene su veće vrijednosti imunoglobulina u kolostrumu krava u odnosu na prvotelke te u starijih krava u odnosu na mlađe krave. Simentalske krave imale su veću koncentraciju imunoglobulina u kolostrumu u odnosu na holštajn krave i križanke, a najveće vrijednosti imunoglobulina zabilježene su tijekom zimskih mjeseci, dok su najmanje vrijednosti bile tijekom proljetnog razdoblja.

Abstract (english)

Calves are born without protective substances (immunoglobulin) which would protect them from different pathogens. So, it is very important that calves are fed with high-quality colostrum rich in immunoglobulin. The aim of this master thesis was to determine immunoglobulin concentration in colostrum, and to determine effect of age, lactation, season and breed on immunoglobulin concentration. The concentration of immunoglobulin was measured using two devices: colostrum densimeter and refractometer. Average immunoglobulin concentration measured with colostrum densimeter during first milking was 68.19 mg/mL, while measured with refractometer was 56.31 mg/mL. Measured concentrations were higher than threshold values (50 mg/mL) meaning that the average colostrums immunoglobulin concentration on family farm Hubak was satisfactory. High positive correlation (R2=0.80; P<0.0001) was found between measurements with two different devices. Due to that, both devices can be used with same reliability for measuring immunoglobulin concentration in cattle colostrums. Significant effect of age, parity, season and breed on colostrum immunoglobulin concentration was not found. However, higher immunoglobulin concentration was found in colostrum of cows in later parities and first-calved heifers i.e. older than younger cows. Simmental cows had higher immunoglobulin concentration than Holstein cows or their crossbreds. The highest immunoglobulin concentrations were found during winter months, while the lowest were during spring period.

• Keywords: kvaliteta kolostruma; imunoglobulini; laktacija; redoslijed laktacije; sezona; pasmina
• Keywords (english): colostrum quality; immunoglobulins; lactation; number of lactacion; season; breed
• Language: croatian
• URN:NBN: urn:nbn:hr:204:329203
• Study programme: Title: Production and Processing of Milk; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka proizvodnje i prerade mlijeka (magistar / magistra inženjer/inženjerka proizvodnje i prerade mlijeka)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-06-21 11:43:07