Retestiranje matičnog nasada klonskih kandidata dalmatinskih sorata vinove loze na prisustvo/pojavnost virusa

master's thesis

• Title (english): Retesting of mother vineyard of grapevine clonal candidates dalmatian varieties on virus presence
• Author: Martin Kovačević
• Mentor: Edi Maletić (mentor)
• Committee member: Edi Maletić (predsjednik povjerenstva)
• Committee member: Darko Preiner (član povjerenstva)
• Committee member: Darko Vončina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Viticulture and Enology); Zagreb
• Defense date and country: 2018-05-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Pokusni nasad Baštica podignut je 2008. godine s ciljem provođenja individualne klonske selekcije sedam najvažnijih dalmatinskih autohtonih sorata: Plavac mali, Plavina, Pošip, Vugava, Maraština, Grk i Debit. U nasad je posađeno vegetativno potomstvo bezvirusnih matičnih trsova navedenih sorata izdvojenih u masovnoj pozitivnoj selekciji provedenoj na području Dalmacije u 2006. godini koji su predstavljali klonske kandidate. S obzirom na sanitarni status predmetnih sorata tj. klonskih kandidata pokusni nasad ujedno je registriran kao prvi certificirani matični nasad ovih sorata te je uz provođenje istraživanja svojstava klonskih kandidata koristio se i za dobivanje jedinih količina certificiranih plemki predmetnih sorata za rasadničarsku proizvodnju. Nakon osam godina od podizanja i izdvajanja najboljih klonskih kandidata uočena je i prva pojava vizualnih simptoma koji su upućivali na mogućnost reinfekcije virusom uvijanja lista (GLRaV-3) kod određenog broja trsova u nasadu. Cilj ovog istraživanja je bio utvrditi zdravstveno stanje izdvojenih klonova sedam autohtonih dalmatinskih sorata nakon provedene individualne klonske selekcije s ciljem proizvodnje predbaznog sadnog materijala i njihove registracije i time dobiti uvid i u sanitarni status samog matičnog nasada. Nakon prikupljanja uzoraka i provedenog testiranja na Zavodu za fitopatologiju Agronomskog fakulteta u Zagrebu, sumnje u prisutnost gospodarski štetnih virusa bile su opravdane. Kod svake testirane sorte (Maraština, Pošip, Grk, Vugava, Debit, Plavina i Plavac mali ) pronađena je prisutnost virusa barem na jednom od izdvojenih klonova. Unatoč tome što je utvrđen dovoljan broj zdravih trsova kod većine izabranih klonova predmetnih sorata (osim Grka) za proizvodnju predbaznog sadnog materijala i njihovu registraciju, potvrđeno je da sam matičnjak „Baštica“ više nije pogodan za proizvodnju certificiranih plemki ovih sorata.

Abstract (english)

Experimental vineyard Bastica was planted in 2008. The intention was to carry out clonal selection of seven native Dalmatian varieties. In vineyard was planted vegetative progeny of the non-viral rootstocks of the mentioned varieties separated as a result of the mass positive selection in Dalmatia in 2006. Concerning the sanitary status of clone candidates, the experimental vineyard was also registered as the first certified mother vineyard of this varieties. During the researches of the characteristics of clone candidates, it was also used for providing the only certified material of the varieties for the nursery production. Eight years after raising the vineyard and isolating the best candidates, the first appearance of visual symptoms that led to the possibility of reinfection with GLRaV-3 (Grapevine Leafroll associated Virus) was observed. The aim of the study was to determine the sanitary status of the clones of the seven native Dalmatian varieties in order to start production of certified planting material. For that purpose presence of viruses was screened by ELISA method in mother vineyard Bastica on clone candidates of certain clones belonging to seven indigenous Dalmatian varieties . After collecting samples in mother vineyard of clonal candidates and performing ELISA at the Department of Plant Pathology at University of Zagreb Faculty of Agriculture, doubts about the presence of economic viruses were justified. At each tested variety (Marastina, Posip, Grk, Vugava, Debit, Plavina and Plavac mali) virus presence was found at least in one of the selected clones. Despite the fact that that a sufficient number of healthy grapes was established (except Grk) in the most of the selected clone candidates, it is confirmed that mother vineyard Bastica is no longer suitable for the production of certified material.

• Keywords: autohtone sorte; klonovi; metoda ELISA; certificirani sadni materijal
• Keywords (english): autochthonous varieties; clones; ELISA; certified planting material
• Language: croatian
• URN:NBN: urn:nbn:hr:204:875740
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-06-06 07:49:33