Anatomske i mikromorfološke karakteristike tepala tulipana

master's thesis

• Author: Kristina Babić
• Mentor: Tatjana Prebeg (mentor)
• Committee member: Tatjana Prebeg (član povjerenstva)
• Committee member: Vesna Židovec (član povjerenstva)
• Committee member: Dubravka Dujmović Purgar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Ornamental Plants, Landscape Architecture and Garden Art); Zagreb
• Defense date and country: 2016-03-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy; Ornamental Plants

Abstract

Istraživanja anatomskih i mikromorfoloških karakteristika tepala tulipana (Tulipa gesneriana L.) provedena su na četiri kultivara različitih boja cvjetova ('Apeldoorn's Elite', 'Atlantis', 'Groenland' i 'Queen of the Night'). Tepali istraženih kultivara su po svojoj anatomskoj građi bili vrlo slični. Anatomske karakteristike unutarnjih i vanjskih tepala se također nisu bitno razlikovale. Epidermske stanice bile su izdužene u smjeru dulje osi tepala te su imale ravne vanjske stijenke. Bočne i unutarnje stijenke su također većinom bile ravne, osim u vršnom dijelu tepala, gdje je kod tri istražena kultivara ('Apeldoorn's Elite', 'Atlantis', 'Queen of the Night') bio vidljiv niz izbočina. Kod svih su kultivara bili jasno vidljivi nabori kutikule koji su se uglavnom pružali u smjeru dulje osi stanica, no bilo je i onih čiji je smjer bio okomit na dulju os. Iako su nabori bili više ili manje ravni ili valoviti, iridescencija je bila vidljiva samo na abaksijalnoj strani tepala kultivara 'Queen of the Night'. Stanice mezofila također su bile izdužene u smjeru dulje osi tepala te prilično gusto raspoređene, osim u vršnom dijelu tepala, gdje je mezofil bio rahlije građe. Boju tepalima davale su tri skupine pigmenata: antocijani, karotenoidi, a kod kultivara 'Groenland' i klorofili. Antocijani su redovito bili prisutni samo u vakuolama epidermskih stanica. Karotenoidi, pohranjeni u kromoplastima, u najvećoj su se količini nalazili u epidermskim stanicama, i u njima su dolazili zasebno ili u kombinaciji s antocijanima. Kromoplasti su, osim u epidermi, u manjoj količini bili prisutni i u subepidermi, dok su prema središnjem dijelu mezofila bili sve rjeđi. U radu je diskutiran utjecaj morfoloških karakteristika epidermskih stanica i nabora kutikule na boju i izgled površine tepala.

Abstract (english)

Anatomical and micromorphological characteristics of tulip (Tulipa gesneriana L.) tepals were studied on four cultivars with different colors of flowers ('Apeldoorn's Elite', 'Atlantis', 'Groenland' and 'Queen of the Night'). Anatomical characteristics of tepals were very similar in all investigated cultivars. Also, the anatomical features of inner tepals did not significantly differ from those in outer tepals. Epidermal cells were elongated in the direction of the longer axis of tepals and their outer walls were flat. Side and inner cell walls had also been mostly flat, except in the periphery of the tepals in which series of protrusions were visible in three cultivars ('Apeldoorn's Elite', 'Atlantis', 'Queen of the Night'). All cultivars showed clearly visible cuticle striations which mostly spread in the direction of the longer axis of the cells, though in some cases they were perpendicular. Although the striations were more or less straight or wavy, iridescence was visible only in the abaxial side of tepals in cultivar 'Queen of the Night'. Mesophyll cells were also elongated in the direction of the longer axis of the tepals and densely packed, except in peripheral region of tepals, where mesophyll cells were more loosely arranged. Tepals were colored by three groups of pigments: anthocyanins, carotenoids and, in cultivar 'Groenland', chlorophylls. Anthocyanins were present only in the vacuoles of epidermal cells. Carotenoids, deposited in chromoplasts, were mostly found in epidermal cells, in which they were present alone or in combination with anthocyanins. Chromoplasts were also found in subepidermis and became more rare towards the central part of mesophyll. Possible effects of morphological characteristics of epidermal cells and cuticle striations on tepal color and texture are discussed.

• Keywords: tulipan; epidermske stanice; kutikula; antocijani; karotenoidi
• Keywords (english): tulips; epidermal cells; cuticule; anthocyanins; carotenoids
• Language: croatian
• URN:NBN: urn:nbn:hr:204:741613
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-05-16 10:43:13