Utvrđivanje vrste Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975 u uzorcima tla iz lokaliteta Belica

master's thesis

• Title (english): Identification of species Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975 in soil samples from the locality of Belica
• Author: Matea Pinturić
• Mentor: Dinka Grubišić (mentor)
• Committee member: Dinka Grubišić (predsjednik povjerenstva)
• Committee member: Tanja Gotlin Čuljak (član povjerenstva)
• Committee member: Ivanka Žutić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Agricultural Zoology); Zagreb
• Defense date and country: 2016-03-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy; Phytomedicine

Abstract

Zlatna krumpirova cistolika nematoda – Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975 ubraja se među najvažnije štetne organizme krumpira u svijetu. Prvi nalaz vrste G. rostochiensis u Republici Hrvatskoj zabilježen je 2001. godine na području Belice u Međimurskoj županiji (Oštrec i Grubišić, 2002). Zaraza je utvrđena na poljima gdje je krumpir uzgajan u monokulturi. Od tada se provodi sustavno praćenje pojave i raširenosti krumpirovih cistolikih nematoda u Republici Hrvatskoj. Kako je analizom uzoraka tla određena prisutnost zlatnožute krumpirove cistolike nematode, istraživanja su nastavljena i sljedećih godina na cijelom području Republike Hrvatske. Do danas je vrsta G. rostochiensis utvrđena na pojedinim lokalitetima u Međimurskoj, Varaždinskoj, Zagrebačkoj i Primorsko-goranskoj županiji.
U ovom radu prikazani su rezultati istraživanja prisutnosti vrste G. rostichiensis u uzorcima tla prikupljenim 2003. g. na lokalitetu Belica u Međimurskoj županiji. Cilj rada bio je utvrditi intenzitet zaraze i visinu populacije te vrste brojanjem vitalnih cista izdvojenih iz uzoraka tla. Osušeni uzorci tla podvrgnuti su postupku izdvajanja cistolikih nematoda metodom flotacije, Spearsovim flotacionim uređajem, a intenzitet zaraze određen je s obzirom na brojnost cista u 100 ml tla. Prisutnost zlatne krumpirove nematode utvrđena je u svih pet analiziranih uzoraka tla, a prosječan broj cista iznosio je 434 ciste/100 ml tla. Prosječan broj vitalnih cista iznosio je 93,41 jaja i ličinki/g tla čime je prekoračen ekonomski prag štetnosti od 20 jaja i ličinki/g tla. Zbog visoke populacije tog karantenskog štetnika na uzorkovanoj površini, potrebno je provoditi mjere integrirane zaštite krumpira od krumpirovih cistolikih nematoda što uključuje: plodored, sadnju otpornih kultivara, sterilizaciju tla, „trap cropping“ te primjenu nematocida.

Abstract (english)

Golden potato cysts nematode – Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975 is among the most harmful organisms of potatoes in the world. First discovery of G. rostochiensis in Croatia was in Belica in Međimurje county in the year 2001 (Oštrec and Grubišić, 2002). Disease is determined in the fields on which potato was grown as monoculture. Since then it has been carried out systematic monitoring of the occurrence and spread of potato cyst nematodes in the Republic of Croatia. As the analysis of these samples determined the presence of golden potato cyst nematode, research has continued in the following years on the whole Croatian territory. To date, G. rostochiensis was found in some locations in Međimurje, Varaždin, Zagreb and Primorsko-goranska county.
This paper presents the results of the presence of G. rostochiensis in soil samples collected in 2003, on the locality of Belica in Međimurje county. The aim of this study was to determine the intensity of infection and the population height by counting vital cysts isolated from soil samples. The dried soil samples were subjected to the process of separating cyst nematodes by flotation method in Spears flotation device. Intensity of infection was determined by the number of cysts in 100 ml of soil. The presence of golden potato nematode was found in all of five analyzed soil samples, and the average number of cysts was 434 cysts/100 ml of soil. The average number of vital cyst was 93,41 eggs and larvae/g soil which exceeded economic thresholds of 20 eggs and larvae/g soil. Due to the high population of that quarantine pest on the sampled surface, it is necessary to implement measures of integrated pest management including: crop rotation, planting resistant cultivars, soil sterilization, trap cropping, and nematocide application.

• Keywords: Zlatna krumpirova cistolika nematoda; uzorkovanje tla; Spearsov flotacioni uređaj; vitalnost cista
• Keywords (english): Golden potato cyst nematode; soil sampling; Spears flotation device; cysts vitality
• Language: croatian
• URN:NBN: urn:nbn:hr:204:905943
• Study programme: Title: Phytomedicine; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-05-11 09:20:56