Utjecaj suvišne vlažnosti na fenotipske karakteristike simbioznih fiksatora dušika izoliranih iz kvržica soje

master's thesis

• Author: Ivana Ćurčić
• Mentor: Mihaela Blažinkov (mentor)
• Committee member: Mihaela Blažinkov (predsjednik povjerenstva)
• Committee member: Sanja Sikora (član povjerenstva)
• Committee member: Ana Pospišil (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Microbiology); Zagreb
• Defense date and country: 2015-07-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy; Crop Production

Abstract

Za poljoprivrednu proizvodnju vrlo je značajna simbioza kvržičnih bakterija i mahunarki. Stoga je važno odabrati visoko učinkovite sojeve koji su otporni na razne nepovoljne uvjete u tlu. Zato je selekcija visoko učinkovitih sojeva kvržičnih bakterija bitna za uspješnost predsjetvene bakterizacije sjemena mahunarki jer se danas sve više susrećemo s ekstremnim vremenskim pojavama koje su posljedica klimatskih promjena. Glavni cilj rada je utvrditi fenotipske karakteristike sojeva kvržičnih bakterija izoliranih iz kvržica soje sa poplavljenih područja županjske Posavine u uvjetima koji su izvan optimalnih vrijednosti za njihov rast. Rast sojeva istraživan je na različitim pH vrijednostima, temperaturama, koncentracijama soli i teških metala te izvorima ugljikohidrata. Istraživanjem je utvrđeno da ispitivani izolati kvržičnih bakterija mogu rasti na temperaturi od 37 C koja je viša od njihove optimalne temperature (26 – 30 C). Većina ispitanih izolata kvržičnih bakterija koji inače imaju najbolji rast u neutralnim sredinama, toleriraju lužnate i kisele sredine. Istraživanje je pokazalo da izolati ne toleriraju povećane koncentracije soli i ne toleriraju rast na podlogama s bakrom. Pokazali au dobar rast na drugim izvorima ugljikohidrata osim glukoze i dobar rast na podlogama s cinkom i manganom dok su na podlogama s kadmijem pokazali djelomičan rast.

Abstract (english)

For agricultural production symbiosis of nodule bacteria and legumes is very important. Therefore, it is important to choose the highly effective strains that are resistant to various unfavorable conditions in the soil. The selection of highly efficient strains of rhizobia is essential to the success of legume seed inoculation because today we are increasingly faced with extreme weather phenomena due to the climate change. The aim of this thesis is to determine the phenotypic characteristic of nodule bacteria strains isolated from soybean nodules from a flooded area of Županja in Posavina in conditions that are beyond the optimum value for their growth. The growth of the strains was studyed at different pH values, temperatures, concentrations of salts and heavy metals, and carbohydrate sources. It was found that the studied isolates of nodule bacteria can grow at temerature of 37 C which is higher than their optimum temperature (26 – 30 C). Most of the tested nodule bacteria isolates, than normally have the best growth in the neutral media, tolerate alkaline and acidic environment. The study showed that isolates do not tolerate the increased concentration of salt and do not grow on the substrates with copper. They showed good growth in other sources of carbohydrates apart from glucose, and on the surfaces with zinc and manganese, while on the surfaces that contain cadmium they showed a partial growth.

• Keywords: biološka fiksacija dušika; fenotipska karakteristika sojeva; soja (Iglycine max L.); kvržične bakterije
• Keywords (english): biological nitrogen fixation; phenotyoic characteristic of strains; soybean (Glycine max L.). nodule bacteria
• Language: croatian
• URN:NBN: urn:nbn:hr:204:580718
• Study programme: Title: Biljne znanosti; Study programme type: sveučilišni; Study level: diplomski; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Restricted access
• Created on: 2016-05-04 12:55:20