Comparsion of different methods for determining seed viability of Ambrosia artemisiifolia L.

master's thesis

• Title: Usporedba različitih metoda za utvrđivanje vijabilnosti sjemena korovne vrste Ambrosia artemisiifolia L.
• Author: Matej Batinić
• Mentor: Maja Šćepanović (mentor)
• Committee member: Maja Šćepanović (predsjednik povjerenstva)
• Committee member: Milan Poljak (član povjerenstva)
• Committee member: Klara Barić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Herbology); Zagreb
• Defense date and country: 2017-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Ambrosia artemisiifolia L. agresivna korovna je vrsta zbog čijih se prvenstveno alergenih svojstava proučavaju različite mogućnosti prognoze nicanja i suzbijanja. Analizom banke sjemena tla može pomoći boljem razumijevanju dinamike populacije ambrozije što dovodi do učinkovitijeg suzbijanja ove vrste. Za procjenu dinamike nicanja ambrozije potrebno je utvrditi udio dormantnog, nedormantnog (klijavog) i mrtvog sjemena u sjemenskoj populaciji. To je moguće obaviti pomoću različitih nedestruktivnih i destruktivnih metoda utvrđivanja vijabilnosti sjemena. Cilj ovog diplomskog rada bio je: I) usporediti rezultate utvrđivanja vijabilnosti sjemena ambrozije trima destruktivnim metodama: testom klijavosti, tetrazolium testom i crush testom, te II) utvrditi da li između tetrazolium i crush testa postoje razlike u pouzdanosti, kao i mogućnost ponovljivosti istih. Rezultati istraživanja ukazuju da nema znatne razlike u vijabilnosti sjemena ambrozije između tetrazolium testa, crush testa te testa klijavosti upotpunjenim s tetrazolium testom. Kod sve tri metode utvrđeno je oko 90% vijabilnog sjemena. Testom klijavosti utvrđeno je 77% klijavih sjemenki stoga za utvrđivanje vijabilnih sjemenki potrebno ga je upotpuniti s tetrazolium ili crush testom. Subjektivna procjena vijabilnosti prve ocjene TTC testa istog ocjenjivača u vremenskom odmaku od 60 minuta u odnosu na drugu ocjenu, nije znatno promijenjena. Rezultati usporedbe prve i druge ocjene oba ocjenjivača ukazuju da su na sličan način procjenjivali vijabilnost sjemena što potvrđuje pouzdanost i ponovljivosti TTC testa. Crush test također daje objektivne rezultate vijabilnosti sjemena ambrozije, te njime nije utvrđena značajna razlika u procjeni vijabilnosti sjemena ambrozije.

Abstract (english)

Ambrosia artemisiifolia L. is an aggressive weed species whose predominantly allergenic properties are studied by various possibilities of prognostication and suppression. Analyzing the soil seed bank can help to better understand the dynamics of the Ambrosia population, which results in more effective suppression of this species. In order to get accurate data on the dynamics of Ambrosia initiation, it is necessary to determine the percentage of dormant, non-dormant (germinating) and dead seed in the seed population. This can be achieved with different non-destructive and destructive methods which can determine viability of the seed. The aim of this master’s thesis was to: I) compare the results of validation of the seeds viability with three destructive methods: germination test, tetrazolium test and crush test; and II) to determine whether differences between the tetrazolium and the crush test were differences in reliability as well as the possibility of repeatability. The results of research indicate that there is no significant difference in the viability of the Ambrosia seed between tetrazolium test, crush test and germination test supplemented with the tetrazolium test. All three methods identified about 90% of the viability seed. Germination test showed 77% germinating seeds, so it is necessary to supplement it with tetrazolium or crush test to determine viability of the seeds. Visual perception of the same researcher of the first grade rating of the same evaluator in the time lag of 60 minutes on the second grade, is not significantly changed. The results of the comparison of the first and second evaluations of both researcher indicate that they have similarly evaluated seed viability, which confirms the reliability and repeatability of the TTC test. Crush test also gives objective results of Ambrosia seed viability and there is no significant difference in estimation of it.

• Keywords: ambrozija; test klijavosti; crush test; tetrazolium test; vijabilnost sjemena; pouzdanost metoda
• Keywords (english): Ambrosia; germination test; crush test; tetrazolium test; seeds viability; reliability of methods
• Language: croatian
• URN:NBN: urn:nbn:hr:204:673494
• Study programme: Title: Fitomedicina; Study programme type: sveučilišni; Study level: diplomski; Academic / professional title: magistar/ magistra inženjer/inženjerka fitomedicine (magistar/ magistra inženjer/inženjerka fitomedicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Restricted access
• Created on: 2017-11-27 08:00:15