Praćenje rasta mikroreznica vinove loze u kulturi tkiva

undergraduate thesis

• Title (english): Monitoring the growth of wine microcuttings in tissue culture
• Author: Anđa Madžar
• Mentor: Zvjezdana Marković (mentor)
• Committee member: Zvjezdana Marković (predsjednik povjerenstva)
• Committee member: Domagoj Stupić (član povjerenstva)
• Committee member: Iva Šikuten (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Viticulture and Enology); Zagreb
• Defense date and country: 2024-07-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Vinova loza, kultura iznimnog značaja, proširena u svim dijelovima svijeta, već duži niz godina predmet je istraživanja u in vitro uvjetima radi rješavanja problema koje vinogradarstvo susreće u praksi. Za svaki pojedini genotip traži se metoda koja bi ubrzala multiplikaciju u kontroliranim uvjetima. Uzgoj kulture u in vitro uvjetima znači uzgoj u staklu, drugim riječima, podrazumijeva uzgoj biljaka u kontroliranim uvjetima pod konstantnom temperaturom i vlagom na hranjivim podlogama. Sastav hranjive podloge značajno utječe na rast i razvoj uzgajane kulture. Ovaj rad se temelji na istraživanju utjecaja pojedinih hranjivih podloga na rast mikroreznica vinove loze sorti: Chardonnay, Graševina, Grk i Pošip, te dva genotipa nastala križanjem dviju sorata: GRP‐33 i DRP‐7. Sorte se međusobno razlikuju u brzini rasta mikroreznica u in vitro uvjetima, a MS hranjiva podloga se pokazala pogodnija za rast od CP hranjive podloge. Analizom rezultata utvrđena je signifikantna razlika u visini mikroreznica između genotipova GRP‐33, DRP‐7 i Chardonnay u prvom pokusu, kao i između genotipova Grk, Pošip i Graševina u drugom pokusu. Usporedbom vrijednosti drugog parametra, broja nodija, nema značajnih razlika ni kod jedne od praćenih skupina genotipova.

Abstract (english)

The grapevine, a culture of exceptional importance, expanded in all parts of the world, has been the subject of research in vitro for many years to solve the problems that viticulture encounters in practice. For each individual genotype, a method is sought that would accelerate multiplication under controlled conditions. Cultivation of culture in in vitro conditions means cultivation in glass, in other words, it implies cultivation of plants under controlled conditions under constant temperature and humidity on nutrient media. The composition of the nutrient medium significantly affects the growth and development of the cultivated culture. This work is based on the research of the influence of certain nutrient media on the growth of microcuttings of grapevine varieties: Chardonnay, Graševina, Grk and Pošip, and two genotypes created by crossing two varieties: GRP‐33 and DRP‐7. Varieties differ from each other in the growth rate of microcuttings in in vitro conditions, and the MS nutrient medium proved to be more suitable for growth than the CP nutrient medium. Analysis of the results revealed a significant difference in the height of microcuttings between the genotypes GRP‐33, DRP‐7 and Chardonnay in the first experiment, as well as between the genotypes Grk, Pošip and Graševina in the second experiment. By comparing the values of the second parameter, the number of nodes, there are no significant differences in any of the monitored groups of genotypes.

• Keywords: in vitro; hranjiva podloga; regulator rasta
• Keywords (english): in vitro; nutrient medium; growth regulator
• Language: croatian
• URN:NBN: urn:nbn:hr:204:827213
• Study programme: Title: Horticulture; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka hortikulture (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-14 10:13:29