Smeđa mramorasta stjenica (Halyomorpha halys (Stal, 1855)) kao prijenosnik spora mikotoksigenih gljiva

master's thesis

• Title (english): Brown marmorated stink bug (Halyomorpha halys (Stal, 1855)) as a vector of mycotoxigenic fungal spores
• Author: Tina Butić
• Mentor: Slaven Zjalić (mentor)
• Committee member: Slaven Zjalić (predsjednik povjerenstva)
• Committee member: Tomislav Kos (član povjerenstva)
• Committee member: Ivan Širić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; Zagreb
• Defense date and country: 2024-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Smeđa mramorasta stjenica (Halyomorpha halys) je invazivna vrsta azijskog podrijetla. Osim izravnih šteta, može poslužiti kao potencijalni vektor za prijenos spora mikotoksigenih gljiva. Mikotoksini su sekundarni metaboliti gljiva koji kontaminiraju hranu i stočnu hranu, uzrokujući zdravstvene probleme kod ljudi i životinja te ekonomske gubitke u poljoprivredi. Najznačajniji su aflatoksin B1 i okratoksin A. S ciljem istraživanja potencijala H. halys kao prijenosnika mikotoksigenih spora, prikupljeno je 100 odraslih jedinki na pokusnom polju kukuruza u Osijeku. Uzorci su isprani u destiliranoj vodi, inokulirani na hranjive podloge te inkubirani. Izolirane su čiste kulture gljiva koje su morfološki determinirane. Potencijalni proizvođači mikotoksina inokulirani su na tekuće hranjive podloge, ekstrahirani i dokazani TLC metodom. Rezultati su dokazali potencijal H. halys kao vektora spora gljive Aspergillus flavus, proizvođača aflatoksina B1, što predstavlja rizik od kontaminacije usjeva.

Abstract (english)

The brown marmorated stink bug (Halyomorpha halys) is an invasive species of Asian origin. Besides causing direct damage, it can serve as a potential vector of mycotoxigenic fungal spores. Mycotoxins are secondary fungal metabolites that contaminate food and animal feed, causing health problems in humans and animals and economic losses in agriculture. The most significant mycotoxins are aflatoxin B1 and ochratoxin A. To study the potential of H. halys as a vector of mycotoxigenic fungal spores, 100 adult H. halys were collected from maize test field in Osijek. The samples were washed in distilled water, inoculated on nutrient media and incubated. Pure fungal cultures were isolated and morphologically identified. Potential mycotoxin producers were inoculated in liquid nutrient media, extracted, and confirmed using TLC method. The results confirmed the potential of H. halys as a vector of Aspergillus flavus, a producer of aflatoxin B1, which represents a risk of crop contamination.

• Keywords: Halyomorpha halys; mikotoksini; aflatoksin B1; okratoksin A; Aspergillus flavus
• Keywords (english): Halyomorpha halys; mycotoxins; aflatoxin B1; ochratoxin A; Aspergillus flavus
• Language: croatian
• URN:NBN: urn:nbn:hr:204:915261
• Study programme: Title: Mediterranean Agriculture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka mediteranske poljoprivrede (magistar/magistra inženjer/inženjerka mediteranske poljoprivrede)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2024-10-30 12:45:16