Utjecaj bakterija izoliranih iz poplavljenog tla na razvoj korijenovog sustava kupusa

master's thesis

• Title (english): The impact of bacteria isolated from flooded soil on the development of cabbage root systems
• Author: Nikolina Barišić
• Mentor: Ivana Rajnović (mentor)
• Mentor: Ines Sviličić Petrić (komentor)
• Committee member: Ivana Rajnović (predsjednik povjerenstva)
• Committee member: Nataša Hulak (član povjerenstva)
• Committee member: Boris Lazarević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Microbiology); Zagreb
• Defense date and country: 2024-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

U tlu žive različite bakterije, među kojima i tzv. PGP (eng. Plant Growth Promoting), poznate po svojim sposobnostima da potiču rast biljaka kroz različite biološke mehanizme. U ovom radu identificirano je 30 bakterijskih izolata koji su u prethodnim ispitivanjima pokazali da posjeduju PGP svojstva. Sekvenciranjem 16S rRNA regije utvrđeno je da većina izolata pripada rodovima Bacillus i Pseudomonas. U laboratorijskim uvjetima ispitan je njihov utjecaj na rast korijena kupusa. Statističkom obradom podataka utvrđeno je da je većina izolata imala negativan utjecaj na rast korijena u usporedbi s kontrolnim biljkama koje su rasle bez prisutnosti bakterija. Ovaj rad pokazuje da PGP bakterije mogu izazvati stres kod mladih biljaka, što nepovoljno utječe na njihov rast i razvoj korijenovog sustava.
Potrebna su daljnja istraživanja kako bi se detaljnije razumjeli čimbenici koji utječu na učinkovitost ovih bakterija, s posebnim naglaskom na izbjegavanje negativnih učinaka u ranim fazama razvoja biljaka. To će omogućiti njihovu sigurniju primjenu u održivoj poljoprivredi, s ciljem poboljšanja rasta i otpornosti biljaka.

Abstract (english)

Various bacteria live in the soil, including Plant Growth-Promoting (PGP) bacteria known for their ability to stimulate plant growth through various biological mechanisms. In this thesis, 30 bacterial isolates with previously confirmed PGP properties were identified. Sequencing of the 16S rRNA gene region revealed that most isolates belong to the Bacillus and Pseudomonas genera. Their influence on the growth of cabbage roots was tested under laboratory conditions. Statistical processing of the data revealed that most isolates had a negative effect on root growth, compared to control plants grown without bacteria. This study highlights that PGP bacteria can induce stress in young plants, adversely affecting their root development and overall growth. Further research is needed to better understand the factors influencing the efficiency of PGP bacteria and to prevent potential negative effects during early plant development. This will enable their safer application in sustainable agriculture, aimed at improving plant growth and resilience.

• Keywords: PGP bakterije; Brassica oleracea var. capitata; rizosfera; klimatske promjene
• Keywords (english): PGP bacteria; Brassica oleracea var. capitata; rhizosphere; climate change
• Language: croatian
• URN:NBN: urn:nbn:hr:204:903321
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
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• Created on: 2024-10-25 06:34:53