Detekcija gena otpornosti na bolesti kod sjemenjaka nastalih križanjem autohtonih i otpornih sorata vinove loze

master's thesis

• Title (english): Detection of disease resistance genes in seedlings created by crossing autochthonic and resistant vine varieties
• Author: Andrijana Oreški
• Mentor: Darko Preiner (mentor)
• Committee member: Darko Preiner (predsjednik povjerenstva)
• Committee member: Jasminka Karoglan Kontić (član povjerenstva)
• Committee member: Edi Maletić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Viticulture and Enology); Zagreb
• Defense date and country: 2024-07-03, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Vinova loza jedna je od najstarijih i najrasprostranjenijih voćnih vrsta na svijetu. Plemenita vinova loza (Vitis Vinifera L.) nema izražene gene za otpornost što je karakterizira kao vrlo osjetljivu na ekonomski značajne gljivične bolesti kao što su pepelnica (Erysiphe necator syn. Uncinula necator, anamorf Oidium tuckeri) i plamenjača (Plasmopara viticola). Oplemenjivački program Agronomskog fakulteta ima za cilj razvoj otpornih sorata temeljen na autohtonim sortama vinove loze. Ovim istraživanjem nastojalo se utvrditi prisutnost gen lokusa povezanih sa otpornošću na plamenjaču Rpv1, Rpv3, Rpv12 i Run1, Ren3 i Ren9. Run1/Rpv1 lokusi koji se vezano nasljeđuju nisu utvrđeni ni u jednom od istraživanih genotipova. Ostali lokusi potvrđeni su kod značajnog broja križanaca, pri čemu je najzastupljeniji bio Rpv12 lokus utvrđen kod svih križanaca. Istraživanjem je prvi put potvrđena prisutnost Ren9 lokusa u sorti 'Panonia' , a s obzirom da je i kod drugog genotipa korištenog kao izvora otpornosti utvrđena prisutnost lokusa Rpv3, Ren3 i Ren9, postignuto je i homozigotno stanje ovih gen lokusa kod šest analiziranih križanaca.

Abstract (english)

The grape is one of the world's oldest and most widely grown fruit varieties. The noble grape variety (Vitis Vinifera L.) does not have a pronounced resistance gene which characterizes it as highly susceptible to economically significant fungal diseases such as ash borer (Erysiphe necator syn. Uncinula necator, anamorph Oidium tuckeri) and downy mildew (Plasmopara viticola). The storage conditions and stability of heat treatment are indicated in the directions for the additives and premixtures. The breeding program of the Faculty of Agronomy aims at the development of resistant varieties based on native grapevine varieties. This study aims to determine the presence of resistance-related gene loci on flammable Rpv1, Rpv3, Rpv12, and Run1, Ren3, and Ren9. Run1/Rpv1 loci which are linked, have not been identified in any of the studied genotypes. Other loci have been confirmed in many genotypes, the most common being the Rpv12 locus found in all genotypes. The study confirmed for the first time the presence of the Ren9 locus in the variety 'Panonia' and, since the presence of the Rpv3, Ren3, and Ren9 locus was also detected in the second genotype used as a source of resistance, a homozygous state of these locus genes was also achieved in the six genotypes.

• Keywords: Vinova loza; oplemenjivanje; MAS - Marker assisted selection; molekularni markeri; plamenjača; pepelnica
• Keywords (english): Grapevine; breeding; MAS - Marker-assisted selection; molecular markers; downy mildew; powdery mildew
• Language: croatian
• URN:NBN: urn:nbn:hr:204:224556
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2024-07-10 09:18:54