Fenotipska karakterizacija autohtonih sojeva rizobija koje noduliraju soju

Scientific paper - Original scientific paper

• Title (english): Phenotypic characterization of indigenous soybean rhizobia
• Author: Sanja Kajić
• Author: Nika Skendrović
• Author: Ivana Rajnović
• Author: Sanja Sikora
• Author's institution: University of Zagreb; Faculty of Agriculture
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract (croatian)

Kvržične bakterije u simbioznim zajednicama s mahunarkama omogućuju značajan unos biološki vezanog dušika u tlo. Veliku važnost ima odabir sojeva koji su otporni na stresne uvjete u tlu. Danas se sve više susrećemo s ekstremnim vremenskim uvjetima te je od iznimne važnosti selekcija visoko učinkovitih sojeva kvržičnih bakterija (rizobija) kako bi predsjetvena bakterizacija sjemena mahunarki bila uspješna. Cilj ovog istraživanja bio je utvrditi fenotipske karakteristike sojeva kvržičnih bakterija izoliranih iz kvržica soje u uvjetima koji su izvan optimalnih vrijednosti za njihov rast. Uzorci tla za izolaciju sojeva prikupljeni su s područja Koprivničko-križevačke, Bjelovarsko-bilogorske i Vukovarsko- srijemske županije. Sposobnost rasta sojeva ispitivana je na različitim pH vrijednostima, temperaturama, koncentracijama soli, izvorima ugljikohidrata te je testirana osjetljivost na antibiotike. U svrhu utvrđivanja biokemijskog statusa svakog pojedinog izolata provedena su tri biokemijska testa (oksidaza, ureaza i katalaza) te su istražene morfološke karakteristike izolata. Utvrđeno je da je većina autohtonih sojeva rizobija iz ovog istraživanja u in vitro uvjetima pokazala značajnu otpornost na ispitivane uvjete, ali i da se međusobno značajno razlikuju po određenim svojstvima. Izolati K2 i K3 iz Koprivničko-križevačke županije pokazali su najveću tolerantnost na povišene koncentracije soli. Od ispitivanih antibiotika, ampicilin je imao najslabiji utjecaj na izolate, dok su na streptomicin izolati bili najosjetljiviji. Dobiveni rezultati ukazuju na značaj selekcije autohtonih sojeva rizobija.

Abstract (english)

Nodule bacteria in symbiotic communities with legumes allow a significant uptake of biologically fixed nitrogen into the soil. The selection of strains that are resistant to stressful soil conditions is of great importance. Selection of highly efficient strains of nodule bacteria (rhizobia) for successful pre-sowing bacterization of legume seed is especially important today, when we are increasingly faced with extreme weather conditions. The aim of this study was to determine the phenotypic characteristics of strains of nodule bacteria isolated from soybean nodules under conditions that are beyond the optimal values for their growth. Soil samples for strain isolation were collected from Koprivnica-Križevci, Bjelovar- Bilogora and Vukovar-Srijem counties. The ability of strains to grow was tested at different pH values, temperatures, salt concentrations and carbohydrate sources. Antibiotic susceptibility was tested as well. In order to determine the biochemical status of each individual isolate, three biochemical tests (oxidase, urease and catalase) were performed, and the morphological characteristics of the isolates were investigated. It was found that most indigenous strains of rhizobia from this study in vitro showed significant resistance to testing conditions, but they also differ significantly from each other in certain properties. Isolates K2 and K3 from Koprivnica-Križevci county showed the highest tolerance to elevated salt concentrations. From tested antibiotics, ampicillin showed to have the weakest effect on the selected isolates, while the isolates were most sensitive to streptomycin. The obtained results indicate the importance of selection of autochthonous strains of rhizobia.

• Keywords (croatian): soja (Glycine max L.); kvržične bakterije; fenotipska karakterizacija sojeva; biokemijski testovi
• Keywords (english): soybean (Glycine max L.); nodule bacteria; phenotypic characterization of strains; biochemical tests
• Language: croatian
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Glasnik Zaštite Bilja
• Numbering: vol. 44., no. 3., pp. 18-24
• p-ISSN: 0350-9664
• e-ISSN: 2584-3265
• DOI: https://doi.org/10.31727/gzb.44.3.3
• URN:NBN: urn:nbn:hr:204:857024
• Publication: 2021
• Document URL: https://hrcak.srce.hr/257076
• Type of resource: Text
• Access conditions: Open access
• Created on: 2024-02-09 07:10:57