Kvantifikacija toksičnog učinka aluminija kod kukuruza primjenom multispektralnih analiza

master's thesis

• Title (english): Quantification of the aluminium toxicity in maize using multispectral analysis
• Author: Mirna Strugar
• Mentor: Boris Lazarević (mentor)
• Committee member: Boris Lazarević (predsjednik povjerenstva)
• Committee member: Hrvoje Kutnjak (član povjerenstva)
• Committee member: Vedran Rubinić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Nutrition); Zagreb
• Defense date and country: 2023-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Aluminij (Al) je treći najzastupljeniji element u Zemljinoj kori, te se njegovo otapanje povećava
pri kiselom pH. Kisela tla zauzimaju veliki dio svjetskih obradivih tala, a pri pHH2O nižem od 5,5
aluminij postaje toksičan za biljke. Uzgoj kukuruza (Zea mays L.) na kiselom tlu može biti uzrok
smanjenja prinosa za više od 50 %. Toksičnost Al3+ očituje se inhibicijom rasta korijenovog
sustava, ali se simptomi mogu uočiti i na nadzemnim organima. Cilj rada bio je utvrditi
fiziološke promjene nadzemnih organa kukuruza uzgajanog u uvjetima toksičnih koncentracija
aluminija korištenjem nedestruktivnih metoda multispektralnih analiza. Istraživanje je
provedeno u kontroliranim uvjetima komora rasta, na ukupno 50 biljaka uzgajanih u hranjivim
otopinama s različitim koncentracijama aluminija (0, 50, 100, 200 i 400 µM dm-3 AlCl3).
Multispektralno snimanje svake biljke je provedeno nakon 13 dana uzgoja u tretmanima,
pomoću uređaja CropReporter® (PhenoVation, Wageningen, the Netherlands). U tretmanima
sa aluminijem u koncentracijama od 100 µM dm-3, 200 µM dm-3 i 400 µM dm-3 vidljiv je pad
vegetacijskih indeksa koji ukazuju na izravni ili neizravni toksični učinak aluminija na
fotosintetske pigmente. Pad sadržaja fotosintetskih pigmenata kao posljedicu ima smanjenu
apsorpciju svijetlosti. S porastom koncentracije aluminija rasla je i refleksija u RRed, RGreen, RBlue,
RSpcGrn, te RFarRed spektrima, što ukazuje da u uvjetima toksičnosti aluminija dolazi do smanjenja
usvajanja svjetlosne energije, što će posljedično dovesti do smanjenja fotosintetske aktivnosti.
Ovaj diplomski rad ukazuje na mogućnosti korištenja multispektralnih analiza u svrhu
kvantifikacije fenotipskih promjena nadzemnih organa kukuruza u uvijetima toksičnosti
aluminija.

Abstract (english)

Aluminum (Al) is the third most abundant element in the Earth's crust, and its dissolution
increases at acidic pH. Acidic soils occupy a large part of the world's arable soils, and aluminum
toxicity occurs at a pHH2O lower than 5,5. Growing corn (Zea mays L.) on acidic soil can cause
yield reductions of more than 50%. The toxicity of Al3+ is manifested by the inhibition of the
growth of the root system, but the symptoms can also be observed on the above-ground
organs. The aim of the work was to determine the physiological changes in the above-ground
organs of corn grown under conditions of toxic concentrations of aluminum using nondestructive
methods of multispectral analysis. The research was conducted in controlled
conditions of growth chambers, on a total of 50 plants grown in nutrient solutions with
different concentrations of aluminum (0, 50, 100, 200 and 400 µM dm-3 AlCl3). Multispectral
imaging of each plant was performed after 13 days of cultivation in the treatments, using the
CropReporter® device (PhenoVation, Wageningen, the Netherlands). Treatments with
aluminum in concentrations of 100 µM dm-3, 200 µM dm-3 and 400 µM dm-3, caused reduction
of vegetation indices is visible, which indicates direct or indirect toxic effects of Al on the
photosynthetic pigments. With the increase in aluminum concentration, the reflection in the
RRed, RGreen, RBlue, RSpcGrn and RFarRed spectra also increased, which indicates that in conditions
of aluminum toxicity there is a decrease in the absorption of light energy, which will
consequently lead to a decrease in photosynthetic activity. This work show the usefulness
multispectral analysis for the purpose of quantifying the phenotypic changes of above-ground
organs of corn under conditions of aluminum toxicity.

• Keywords: aluminij; kukuruz; multispektralne analize; vegetacijski indeksi
• Keywords (english): aluminum; corn; multispectral analysis; vegetation indices
• Language: croatian
• URN:NBN: urn:nbn:hr:204:193755
• Study programme: Title: Agroecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka agroekologije (magistar/ magistra inženjer/inženjerka agroekologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-11-13 10:00:02