Utjecaj predsjetvenog tretmana prolinom na induciranje tolerantnosti na sušu kod pšenice

master's thesis

• Title (english): Effect of seed priming treatment with proline on inducing drought tolerance
• Author: Karla Osmak
• Mentor: Ivanka Habuš Jerčić (mentor)
• Committee member: Ivanka Habuš Jerčić (predsjednik povjerenstva)
• Committee member: Hrvoje Šarčević (član povjerenstva)
• Committee member: Željko Jukić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Department of Plant Breeding Genetics, Biometrics and Experimentation); Zagreb
• Defense date and country: 2023-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Klimatske promjene i abiotski stresovi sve su češći problemi u poljoprivrednoj proizvodnji koji za posljedicu imaju smanjenje dostupne hrane za sve veće svjetsko stanovništvo. Jedan od najčešćih abiotskih stresova je suša koja negativno utječe na biljke mijenjajući morfološke, fiziološke i metaboličke procese. Stoga se novim oplemenjivačkim programima, predsjetvenim tretmanima i drugim postupcima pokušava smanjiti njezin utjecaj na biljke. Cilj ovog rada je bio utvrditi utjecaj predsjetvog tretmana sjemena pšenice s različitim koncetracijama prolina (0, 15 i 25 mM) na klijavost sjemena, rani porast mladih izdanaka, morfološka svojstva te kemijska svojstva (sadržaj prolina, enzimatsku aktivnost superoksid dismutaze i peroksidaze) biljaka u suši. Korištenjem otopina različitog osmotskog potencijala (0, -0,5 i -1,0 MPa) inducirana je suša na šest različitih sorata pšenice (Bc1, Bc2, Bc2, Os1, Os2, Os3). Temeljem provedenih analiza utvrđeno je da je predsjetveni tretman prolinom od 15 mM imao pozitivan učinak na duljinu stablijke, svježu i suhu masu nadzemnog dijela te na smanjenu aktivnost superoksid dismutaze (SOD) i peroksidaze (POX).

Abstract (english)

Climate change and abiotic stresses are increasingly common problems in agricultural production, reducing available food for the ever-increasing world population. Drought is one of the most common abiotic stresses, which negatively affects plants by changing morphological, physiological and metabolic processes. Therefore, new breeding programs, seed priming treatments and other procedures are used to reduce its impact on plants. The aim of this work was to determine the influence of seed priming treatment in wheatwith different concentrations of proline (0, 15 and 25 mM) on seed germination, early growth of young shoots, morphological properties and chemical properties (proline content, enzymatic activity superoxide dismutase and peroxidase) of plants under drought. By using solutions of three different concentrations of different osmotic potential of PEG (0, -0.5 MPa and -1.0 MPa), drought was induced in six different varieties of wheat (Bc1, Bc2, Bc2, Os1, Os2, Os3).Based on the analysis, it was determined that the seed priming treatment with 15 mM proline positively affected stem length, fresh and dry weights of shoots, and the reduced activity of superoxide dismutase (SOD) and peroxidase (POX).

• Keywords: pšenica; suša; predsjetveni tretman; prolin; enzimi stresa
• Keywords (english): wheat; drought; seed priming treatment; proline; stress enzymes
• Language: croatian
• URN:NBN: urn:nbn:hr:204:929659
• Study programme: Title: Plant Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2023-10-12 08:30:51