Usporedba standardizirane INFOGEST in vitro metode probavljivosti i in vivo probave karotenoida u kokoši nesilica

master's thesis

• Title (english): Comparison of standardized infogest in vitro digestibility method and in vivo digestion of carotenoids in laying hens
• Author: Sara Kolar
• Mentor: Kristina Kljak (mentor)
• Committee member: Kristina Kljak (predsjednik povjerenstva)
• Committee member: Goran Kiš (član povjerenstva)
• Committee member: Krešimir Salajpal (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Animal Nutrition); Zagreb
• Defense date and country: 2023-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Jedina standardizirana in vitro metoda probavljivosti je INFOGEST metoda, no do sada
nije ispitano koliko je precizna u određivanju probavljivosti karotenoida. Cilj ovog rada je bio
usporediti rezultate standardizirane in vitro i in vivo metode probavljivosti karotenoida u kokoši
nesilica hranjenih dvjema potpunim krmnim smjesama koje se razlikuju u hibridu kukuruza kao
jedinim izvorom karotenoida. U in vivo pokusu uzorkovani su sadržaji duodenuma, jejunuma,
ileuma i debelog crijeva nesilica, dok je INFOGEST metoda provedena s inkubacijo m od 2,5; 3;
3,5; 4; 4,5 i 5 sati. Usporedivi podatci između in vitro i in vivo metoda utvrđeni su za lutein
kojem su vrijednosti nakon 3; 3,5 i 4,5 sati inkubacije slične vrijednostima u jejunumu (P=0,60),
ileumu (P=0,87) i debelom crijevu (P=0,42). Kod ostalih karotenoida, usporedivi podatci su bili
za probavljivost zeaksantina i u kupnih karotenoida i to između inkubacije nakon 4 sata i
jejunuma (P=0,97 i 0,59). Dobiveni podatci pokazuju da je potrebno dodatno prilagoditi
INFOGEST metodu da bude prikladna za probavljivost karotenoida.

Abstract (english)

The only standardised in vitro digestiblity method is the INFOGEST method, but so far it
has not been tested how accurately it determines the digestibility of carotenoids. The aim of this
research was to compare the results of the INFOGEST method and in vi vo data on the
digestibility of carotenoids in laying hens fed two complete feed mixtures differing in the maize
hybrid as the only source of carotenoids. In the in vivo experiment, the contents of the duodenum,
jejunum, ileum and colon of the hens were sa mpled, while the INFOGEST method was
performed with incubation times of 2.5, 3, 3.5, 4, 4.5 and 5 hours. Comparable da t a between the
in vitro and in vivo methods were obtained for lutein, whose values after 3, 3.5 and 4.5 hours of
incubation were comparable with digestibility in the jejunum (P=0.60), ileum (P=0.87) and colon
(P=0.42). For other carotenoids, comparable da t a were obtained for the digestib i lity of
zeaxanthin and total carotenoids between incubation after 4 hours and digestibility in the jejunu m
(P=0.97 and 0.59). The da t a obtained show that it is necessary to make additional adjustments
to the INFOGEST method to ensure suitability for digestibility of carotenoids.

• Keywords: kukuruz; karotenoidi; probavljivost; INFOGEST metoda; nesilice
• Keywords (english): maize; carotenoids; digestibility; INFOGEST method; laying hens
• Language: croatian
• URN:NBN: urn:nbn:hr:204:495688
• Study programme: Title: Animal Nutrition and Feed Science; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane (magistar/ magistra inženjer/inženjerka hranidbe životinja i hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to authenticated users
• Created on: 2023-09-27 08:48:12