Utjecaj zaraze G-virusom (GVG) i badnavirusom (GBV-1) vinove loze na rast vinove loze u kulturi meristema in vitro

master's thesis

• Title (english): The influence of grapevine G-virus (GVG) and badnavirus (GBV - 1) infection on grapevine growth in meristem culture in vitro
• Author: Antonela Gelenđir
• Mentor: Zvjezdana Marković (mentor)
• Committee member: Zvjezdana Marković (predsjednik povjerenstva)
• Committee member: Darko Vončina (član povjerenstva)
• Committee member: Darko Preiner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Agriculture; (Viticulture and Enology); Zagreb
• Defense date and country: 2023-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Agronomy

Abstract

Vinogradarstvo Hrvatske obuhvaća uzgoj velikog broja sorata vinove loze i bazira se na
uzgoju autohtonih sorata. Problem koji postaje sve izraženiji je njihova velika zaraženost
virusima vinove loze. Istraživanje je provedeno na 10 autohtonih sorata vinove loze
uzorkovanih iz Nacionalne kolekcije autohtonih sorata, kojima je prethodno utvrđena zaraza
jednim ili oba od dva nova virusa vinove loze, G-virusa vinove loze (GVG) i badnavirusa
vinove loze 1 (GBV-1). Koristila se kultura vršnog meristema kao metoda ozdravljenja od
virusa, prilikom čega su izolirani vršni meristemi veličine 1 mm. Statističkom analizom
utvrđena je signifikantna razlika između četiri odabrane sorte koje posjeduju dva ponavljanja
(ˈOskorušicaˈ, ˈTanetova lozaˈ, ˈSilbijanacˈ i ˈPetovkaˈ) s obzirom na % izraslih jedinki nakon 2
tjedna, dok kod jedinki regeneriranih nakon 4 tjedna nije utvrđena signifikantna razlika.
Sanitarni status značajno je utjecao na rast i regeneraciju u kulturi tkiva, odnosno jedinke
zaražene GBV-1 ostvarile su 77,8 % regeneracije, one zaražene GVG 78,9 % regeneracije tj.
sanitarni status signifikantno utječe na % izraslih eksplantata nakon 2 tjedna ali ne i na %
regeneriranih jedinki nakon 4 tjedna od unosa u kulturu tkiva. Mjerenjem visine nakon 5
tjedana, utvrđena je značajna razlika između sorata za dvije kategorije visine. Nakon
postizanja visine, manji postotak biljaka je uspješno aklimatiziran (3,1 %), a vrlo nizak
postotak biljaka je postigao uspješnu eliminaciju virusa iz biljnog tkiva ovom metodom
vršnog meristema (0,9 %).

Abstract (english)

Viticulture in Croatia encompasses the cultivation of a large number of grape varieties
and is based on the cultivation of autochthonous varieties. A problem that is becoming more
and more pronounced is their high infection with vine viruses. The research was carried out
on 10 autochthonous grapevine varieties sampled from the National Collection of
autochthonous varieties, which were previously found to be infected with one or both of
two new grapevine viruses, grapevine virus G (GVG) and grapevine badnavirus 1 (GBV-1).
Apical meristem culture was used as a method of recovery from the virus, during which
apical meristems of 1 mm size were isolated. Statistical analysis revealed a significant
difference between the four selected varieties that have two repetitions (ˈOskorušicaˈ,
ˈTanetova lozaˈ, ˈSilbijanacˈ and ˈPetovkaˈ) with regard to the % of grown individuals after 2
weeks, while no significant difference was found in individuals regenerated after 4 weeks.
Sanitary status significantly influenced growth and regeneration in tissue culture, that is,
individuals infected with GBV-1 achieved 77.8% regeneration, those infected with GVG
78.9% regeneration, respectively sanitary status significantly affects the % of explants grown
after 2 weeks but not on the % of regenerated individuals after 4 weeks of introduction into
tissue culture. By measuring the height after 5 weeks, a significant difference was found
between the cultivars for the two height categories. After reaching height, a smaller
percentage of plants were successfully acclimatized (3.1 %), and a very low percentage of
plants achieved successful virus elimination from plant tissue by this apical meristem
method (0.9 %).

• Keywords: autohtone sorte; virusi vinove loze; kultura vršnog meristem
• Keywords (english): autochthonous grape cultivars; grapevine viruses; apical meristem culture
• Language: croatian
• URN:NBN: urn:nbn:hr:204:451273
• Study programme: Title: Horticulture; Study programme type: university; Study level: graduate; Academic / professional title: magistar/ magistra inženjer/inženjerka hortikulture (magistar/ magistra inženjer/inženjerka hortikulture)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-06-30 09:35:33